Title

Subthalamic Glutamic Acid Decarboxylase Gene Therapy: Changes in Motor Function and Cortical Metabolism

Authors

Marina E. Emborg, University of Wisconsin - Madison
Maren Carbon, Center for Neurosciences, Feinstein Institute for Medical Research
James Holden, University of Wisconsin - Madison
Matthew J. During, University of Auckland
Yilong Ma, Center for Neurosciences, Feinstein Institute for Medical Research
Chengke Tang, Center for Neurosciences, Feinstein Institute for Medical Research
Jeffrey Moirano, University of Wisconsin - Madison
Helen Fitzsimons, University of Auckland
Ben Z. Roitberg, University of Illinois at Chicago
Eray Tuccar, University of Illinois at Chicago
Andrew D. Roberts, Minnesota State University - MankatoFollow
Michael G. Kaplitt, Weill Cornell Medical College
David Eidelberg, Center for Neurosciences, Feinstein Institute for Medical Research

Document Type

Article

Publication Date

2007

Abstract

Parkinson's disease (PD) is associated with increased excitatory activity within the subthalamic nucleus (STN). We sought to inhibit STN output in hemiparkinsonian macaques by transfection with adeno-associated virus (AAV) containing the gene for glutamic acid decarboxylase (GAD). In total, 13 macaques were rendered hemiparkinsonian by right intracarotid 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection. Seven animals were injected with AAV-GAD into the right STN, and six received an AAV gene for green fluorescent protein (GFP). Videotaped motor ratings were performed in a masked fashion on a weekly basis over a 55-week period. At 56 weeks, the animals were scanned with ¹⁸F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Histological examination was performed at the end of the study. No adverse events were observed after STN gene therapy. We found that the clinical rating scores for the two treatment groups had different patterns of change over time (group time interaction, PPP

Department

Physics and Astronomy

Publication Title

Journal of Cerebral Blood Flow & Metabolism

Recommended Citation

M.E. Emborg, M. Carbon, J.E. Holden, M.J. During, Y. Ma, C. Tang, J. Moirano, H. Fitzsimons, B.Z. Roitberg, E. Tuccar, A.D. Roberts, M.G. Kaplitt, and D. Eidelberg. (2007). Subthalamic Glutamic Acid Decarboxylase Gene Therapy: Changes in Motor Function and Cortical Metabolism. Journal of Cerebral Blood Flow & Metabolism, 27, 501-509.

DOI

10.1038/sj.jcbfm.9600364

Link to Publisher Version (DOI)

https://doi.org/10.1038/sj.jcbfm.9600364

Publisher's Copyright and Source

Copyright © 2007 International Society for Cerebral Blood Flow & Metabolism. Article published by Nature Publishing Group in Journal of Cerebral Blood Flow & Metabolism, volume 27, 2007, pages 501-509. Available online on July 12, 2006: https://doi.org/10.1038/sj.jcbfm.9600364