Event Title

Examination of Cardiovascular Development in the Offspring of Maternal SHR Rats with Impaired Aldosterone Secreation During Pregnancy, Using a Remote Monitoring System

Location

CSU 253/4/5

Start Date

5-4-2010 10:00 AM

End Date

5-4-2010 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Penny Knoblch

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

The theory ―fetal origins of adult disease‖ states that adverse maternal conditions can produce fetal effects that increase the likelihood of adult disease. Factors affecting maternal rats such as poor nutrition, reduced uterine or placental blood flow, and, increased adrenal hormone levels, produce small fetuses. These fetuses are prone to developing hypertension (high blood pressure) later in life. Aldosterone, secreted from the adrenal gland, acts on the kidney’s tubules to increase sodium reabsorption, thus raising total body sodium, blood volume, and blood pressure. The goal of this project was to determine the effect of reduced aldosterone levels in maternal rats on the blood pressure of the offspring. Surgery was performed on 8 week old female SHR rats using sterile techniques. The adrenal gland on the right side was carefully removed, and the outer layer of the adrenal gland on the left side was destroyed by freezing it with liquid nitrogen. A sham surgery was also performed on rats by surgically opening and closing the rats in the same way, but leaving the adrenal glands undisturbed. Ten days post surgery, the females were mated with a male rat of the same strain. Once the pups reached 12 weeks of age, three males were randomly selected from each litter for implantation of a telemetry devise that sends blood pressure and heart rate measurements to a receiver attached to a computer. The blood pressures and heart rates will be compared between the offspring of adrenal frozen rats, and the offspring of sham rats.

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Apr 5th, 10:00 AM Apr 5th, 12:00 PM

Examination of Cardiovascular Development in the Offspring of Maternal SHR Rats with Impaired Aldosterone Secreation During Pregnancy, Using a Remote Monitoring System

CSU 253/4/5

The theory ―fetal origins of adult disease‖ states that adverse maternal conditions can produce fetal effects that increase the likelihood of adult disease. Factors affecting maternal rats such as poor nutrition, reduced uterine or placental blood flow, and, increased adrenal hormone levels, produce small fetuses. These fetuses are prone to developing hypertension (high blood pressure) later in life. Aldosterone, secreted from the adrenal gland, acts on the kidney’s tubules to increase sodium reabsorption, thus raising total body sodium, blood volume, and blood pressure. The goal of this project was to determine the effect of reduced aldosterone levels in maternal rats on the blood pressure of the offspring. Surgery was performed on 8 week old female SHR rats using sterile techniques. The adrenal gland on the right side was carefully removed, and the outer layer of the adrenal gland on the left side was destroyed by freezing it with liquid nitrogen. A sham surgery was also performed on rats by surgically opening and closing the rats in the same way, but leaving the adrenal glands undisturbed. Ten days post surgery, the females were mated with a male rat of the same strain. Once the pups reached 12 weeks of age, three males were randomly selected from each litter for implantation of a telemetry devise that sends blood pressure and heart rate measurements to a receiver attached to a computer. The blood pressures and heart rates will be compared between the offspring of adrenal frozen rats, and the offspring of sham rats.

Recommended Citation

Gerbensky, Susan and Crystal Taylor. "Examination of Cardiovascular Development in the Offspring of Maternal SHR Rats with Impaired Aldosterone Secreation During Pregnancy, Using a Remote Monitoring System." Undergraduate Research Symposium, Mankato, MN, April 5, 2010.
http://cornerstone.lib.mnsu.edu/urs/2010/poster-session-A/3