Event Title

Determination of Strontium Incorporation in Bone

Location

CSU 253/4/5

Start Date

4-4-2011 11:00 AM

End Date

4-4-2011 12:30 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Michael Bentley

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Strontium is a bone-seeking, trace element that has been shown through previous experiments to be a

―valuable safe alternative‖ for calcium absorption tests. To determine the replacement of calcium with strontium in C57/B16 mice, mice were given distilled water with 0.15 g strontium chloride per 100 mL for different periods of time. Some of the mice were also given a low calcium chow to reduce the amount of calcium available to them through their environment. Control mice were given distilled water and normal chow, or 0.105 g calcium chloride per 100 mL drinking water with normal or low calcium chow. After being on the specific diets for 3 weeks or longer, the mice were euthanized by CO2 inhalation and dissected to examine their femurs. The long bones were removed and stored in 2.5% glutaraldehyde, buffered with 0.1 M phosphate (pH 7.2) until they were ready for analysis by Energy Dispersive X-ray Spectroscopy (EDS) to determine elemental content of strontium. Early results have shown the presence of strontium in mice tibias. Further analysis of the various diets will lead to a better understanding of the effects of strontium chloride water with or without a low calcium diet.

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Apr 4th, 11:00 AM Apr 4th, 12:30 PM

Determination of Strontium Incorporation in Bone

CSU 253/4/5

Strontium is a bone-seeking, trace element that has been shown through previous experiments to be a

―valuable safe alternative‖ for calcium absorption tests. To determine the replacement of calcium with strontium in C57/B16 mice, mice were given distilled water with 0.15 g strontium chloride per 100 mL for different periods of time. Some of the mice were also given a low calcium chow to reduce the amount of calcium available to them through their environment. Control mice were given distilled water and normal chow, or 0.105 g calcium chloride per 100 mL drinking water with normal or low calcium chow. After being on the specific diets for 3 weeks or longer, the mice were euthanized by CO2 inhalation and dissected to examine their femurs. The long bones were removed and stored in 2.5% glutaraldehyde, buffered with 0.1 M phosphate (pH 7.2) until they were ready for analysis by Energy Dispersive X-ray Spectroscopy (EDS) to determine elemental content of strontium. Early results have shown the presence of strontium in mice tibias. Further analysis of the various diets will lead to a better understanding of the effects of strontium chloride water with or without a low calcium diet.

Recommended Citation

Fischer, Cassandra and Danielle Kral. "Determination of Strontium Incorporation in Bone." Undergraduate Research Symposium, Mankato, MN, April 4, 2011.
http://cornerstone.lib.mnsu.edu/urs/2011/poster-session-B/2