Event Title

Localization of the Novel Polyglutamine Protein KIAA1946- Using A Multi Tissue Western Blot

Location

CSU 253/4/5

Start Date

4-4-2011 1:30 PM

End Date

4-4-2011 3:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Geoffrey Goellner

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Of the many proteins expressed in the human body, nearly 20% contain a repeat component in their primary amino acid sequence. The novel protein KIAA1946 contains one such polyglutamine (polyQ) stretch- which interestingly may also be polymorphic. Repeat polymorphisms are significant because mutations in these sequences well beyond their normal range underlie a number of severe neurodegenerative diseases such as Huntington‘s Disease. As KIAA1946 is completely uncharacterized, we would like to know which tissues in the body express this protein. In a preliminary attempt to determine the tissue location of KIAA1946, we transfected a GFP-tagged version of the protein into COS-7 cells in order to test a newly available commercial KIAA1946 antibody via western blotting. We found that although an antibody against the GFP moiety does indeed recognize the fusion protein, the KIAA1946 antibody detected very little. Thus, the new KIAA1946 antibody is likely very weak- and it is too early to tell if COS-7 cells (or any other tissues) express this novel protein. This data indicates an initial attempt in distinguishing the tissue localization of the novel protein, and provides the groundwork for further investigations including: verification of KIAA1946 tissue localization using the new commercially-available KIAA1946 antibody, and intracellular localization using fluorescent tags and fluorescence microscopy.

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Apr 4th, 1:30 PM Apr 4th, 3:00 PM

Localization of the Novel Polyglutamine Protein KIAA1946- Using A Multi Tissue Western Blot

CSU 253/4/5

Of the many proteins expressed in the human body, nearly 20% contain a repeat component in their primary amino acid sequence. The novel protein KIAA1946 contains one such polyglutamine (polyQ) stretch- which interestingly may also be polymorphic. Repeat polymorphisms are significant because mutations in these sequences well beyond their normal range underlie a number of severe neurodegenerative diseases such as Huntington‘s Disease. As KIAA1946 is completely uncharacterized, we would like to know which tissues in the body express this protein. In a preliminary attempt to determine the tissue location of KIAA1946, we transfected a GFP-tagged version of the protein into COS-7 cells in order to test a newly available commercial KIAA1946 antibody via western blotting. We found that although an antibody against the GFP moiety does indeed recognize the fusion protein, the KIAA1946 antibody detected very little. Thus, the new KIAA1946 antibody is likely very weak- and it is too early to tell if COS-7 cells (or any other tissues) express this novel protein. This data indicates an initial attempt in distinguishing the tissue localization of the novel protein, and provides the groundwork for further investigations including: verification of KIAA1946 tissue localization using the new commercially-available KIAA1946 antibody, and intracellular localization using fluorescent tags and fluorescence microscopy.

Recommended Citation

Kopachek, Toni M.. "Localization of the Novel Polyglutamine Protein KIAA1946- Using A Multi Tissue Western Blot." Undergraduate Research Symposium, Mankato, MN, April 4, 2011.
http://cornerstone.lib.mnsu.edu/urs/2011/poster-session-C/8