Event Title

Gene Regulation of Lip1 in Photorhabdus

Location

CSU Ballroom

Start Date

16-4-2013 10:00 AM

End Date

16-4-2013 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Christopher Conlin

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

In the bacterial genus Photorhabdus, there is a gene, lip1, which encodes the expression of a lipase. A similar gene found in Salmonella is regulated by phosphate limitation. The Lip1 promoter is also found in E. coli and regulated by growth phase of the bacteria. However, it is not known what regulates the lip1 gene in the genus Photorhabdus. The importance of the research is to further our understanding of how the Lip1 promoter works. In my research, I grew the bacteria Photorhabdus with a plasmid for the Lip1 promoter that contained regions for beta-galactosidase expression and Kanamycin resistance. Attempts to move this plasmid into a wild-type background failed so another strain was used which already contained the plasmid. I utilized the beta-galactosidase expression to run simple chromogenic assays of the plasmid under different conditions. The conditions I used were variations in growth phase, glucose and phosphate limitation, and rich versus minimal media. I found that there was no effect of glucose or phosphate limitation on Lip1 expression. There was some indication of a growth phase effect and notable differences in some media used. Lip1 expression was reduced in certain rich media, such as Tryptic Soy Broth, LB Broth, and Brain Heart Infusion Broth.

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Apr 16th, 10:00 AM Apr 16th, 12:00 PM

Gene Regulation of Lip1 in Photorhabdus

CSU Ballroom

In the bacterial genus Photorhabdus, there is a gene, lip1, which encodes the expression of a lipase. A similar gene found in Salmonella is regulated by phosphate limitation. The Lip1 promoter is also found in E. coli and regulated by growth phase of the bacteria. However, it is not known what regulates the lip1 gene in the genus Photorhabdus. The importance of the research is to further our understanding of how the Lip1 promoter works. In my research, I grew the bacteria Photorhabdus with a plasmid for the Lip1 promoter that contained regions for beta-galactosidase expression and Kanamycin resistance. Attempts to move this plasmid into a wild-type background failed so another strain was used which already contained the plasmid. I utilized the beta-galactosidase expression to run simple chromogenic assays of the plasmid under different conditions. The conditions I used were variations in growth phase, glucose and phosphate limitation, and rich versus minimal media. I found that there was no effect of glucose or phosphate limitation on Lip1 expression. There was some indication of a growth phase effect and notable differences in some media used. Lip1 expression was reduced in certain rich media, such as Tryptic Soy Broth, LB Broth, and Brain Heart Infusion Broth.

Recommended Citation

Hanson, Taylour. "Gene Regulation of Lip1 in Photorhabdus." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
http://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/17