Event Title

Effect of Methyl Jasmonate on the Expression of Lipoxygenase 5 in Soybean Leaves

Location

CSU Ballroom

Start Date

16-4-2013 10:00 AM

End Date

16-4-2013 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Lipoxygenases (LOX) are enzymes that catalyze the peroxidation of polyunsaturated fatty acids. Multiple LOX isoenzymes are present in plants. They function in growth and development, senescence, defense against pathogens and pests. Each isoenzyme plays a definite role within the plant at different stages of development. Plants produce jasmonic acid (JA) and methyl jasmonate (MJ), in response to stress such as wounding. The jasmonates play a role in the plant’s response to wounding by stimulating the synthesis of toxins which serve as mediators in defense mechanism. Studies have shown that wounding or treatment with MJ affects the expression of some LOX isoforms. This study investigated the effect of wounding and MJ on the expression of LOX 5 in soybean plants. Soybean plants were grown in a growth chamber. When plants reached the biofoliate stage, leaves in one set of plants were wounded with a hemostat, one set was exposed to MJ, one set was wounded and exposed to MJ and a control set was not treated. Leaves were harvested at 0, 3, 7 and 24 hours after treatment. RNA was isolated using the RNeasy Plant Minikit from Qiagen. Quality and quantity of the RNA was assessed by measuring the absorbance at 260 nm and 280 nm. DNA copies of the isolated mRNA were made by reverse transcription using High-Capacity cDNA Reverse Transcription Kit from Applied Biosystems. The relative quantities of LOX 5 DNAs in the samples were determined using Q-PCR. SYBR green was used to detect the PCR products.

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Apr 16th, 10:00 AM Apr 16th, 12:00 PM

Effect of Methyl Jasmonate on the Expression of Lipoxygenase 5 in Soybean Leaves

CSU Ballroom

Lipoxygenases (LOX) are enzymes that catalyze the peroxidation of polyunsaturated fatty acids. Multiple LOX isoenzymes are present in plants. They function in growth and development, senescence, defense against pathogens and pests. Each isoenzyme plays a definite role within the plant at different stages of development. Plants produce jasmonic acid (JA) and methyl jasmonate (MJ), in response to stress such as wounding. The jasmonates play a role in the plant’s response to wounding by stimulating the synthesis of toxins which serve as mediators in defense mechanism. Studies have shown that wounding or treatment with MJ affects the expression of some LOX isoforms. This study investigated the effect of wounding and MJ on the expression of LOX 5 in soybean plants. Soybean plants were grown in a growth chamber. When plants reached the biofoliate stage, leaves in one set of plants were wounded with a hemostat, one set was exposed to MJ, one set was wounded and exposed to MJ and a control set was not treated. Leaves were harvested at 0, 3, 7 and 24 hours after treatment. RNA was isolated using the RNeasy Plant Minikit from Qiagen. Quality and quantity of the RNA was assessed by measuring the absorbance at 260 nm and 280 nm. DNA copies of the isolated mRNA were made by reverse transcription using High-Capacity cDNA Reverse Transcription Kit from Applied Biosystems. The relative quantities of LOX 5 DNAs in the samples were determined using Q-PCR. SYBR green was used to detect the PCR products.

Recommended Citation

Hossain, Sharmin. "Effect of Methyl Jasmonate on the Expression of Lipoxygenase 5 in Soybean Leaves." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
http://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/36