Event Title

The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine

Location

CSU Ballroom

Start Date

16-4-2013 10:00 AM

End Date

16-4-2013 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Normal cells in the human body grow, divide, and then die very uniformly. If the normal cell’s DNA is damaged, the cell will either repair it or die. Aldehydes have been shown to induce the cell’s ability to repair damaged DNA. The analysis of p-anisaldehyde and 3,4-dimethoxybenzaldehyde’s ability to induce this repair is the goal of this research. The focus of this project was to synthesize possible products of these aldehydes with 2’-deoxyguanosine and 2’-deoxyadenosine. p- Anisaldehyde was allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine in dimethyl sulfoxide at 50OC for 24 hours. 3,4-Dimethoxybenzaldehyde was also allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine under the same conditions. Following the initial reaction 1H NMR spectra were analyzed and the reactions were repeated at 70OC for 48 hours. In addition, a DNA dissolved in Tris buffer was also allowed to be treated with each aldehyde at 40OC for 48 hours. After acid hydrolysis, all reaction mixtures were analyzed by HPLC.

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Apr 16th, 10:00 AM Apr 16th, 12:00 PM

The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine

CSU Ballroom

Normal cells in the human body grow, divide, and then die very uniformly. If the normal cell’s DNA is damaged, the cell will either repair it or die. Aldehydes have been shown to induce the cell’s ability to repair damaged DNA. The analysis of p-anisaldehyde and 3,4-dimethoxybenzaldehyde’s ability to induce this repair is the goal of this research. The focus of this project was to synthesize possible products of these aldehydes with 2’-deoxyguanosine and 2’-deoxyadenosine. p- Anisaldehyde was allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine in dimethyl sulfoxide at 50OC for 24 hours. 3,4-Dimethoxybenzaldehyde was also allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine under the same conditions. Following the initial reaction 1H NMR spectra were analyzed and the reactions were repeated at 70OC for 48 hours. In addition, a DNA dissolved in Tris buffer was also allowed to be treated with each aldehyde at 40OC for 48 hours. After acid hydrolysis, all reaction mixtures were analyzed by HPLC.

Recommended Citation

Bowman, Jenna. "The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
http://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/38