Event Title

Rubidium as Substitute for Potassium in Rat Cardiac Tissue

Location

CSU Ballroom

Start Date

21-4-2014 10:00 AM

End Date

21-4-2014 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Victor Esenabhalu

Mentor's Email Address

victor.esenabhalu@mnsu.edu

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

A normal cell contains a sodium/potassium ATPase pump that functions to maintain a high intracellular level of potassium (K+) inside the cell and high levels of sodium (Na+) outside the cell. Since rubidium (Rb+) shares similar chemical properties with potassium and has a similar atomic radius it can replace potassium in all known processes (Olga Jilkina et al). We used rat hearts as a model to determine and quantify how rubidium will take over as potassium in the sodium/potassium pump. First we used the Langendorff system to infuse the aorta of the rat heart with a buffer solution containing Rubidium Chloride (RbCl) so that it circulates through the coronary arteries. The Langendorff apparatus provides a means to control pH, temperature and pressure of the solutions perfusing the heart. A sequel to this procedure is the addition of ouabain to the buffer solution, which will inhibit the sodium/potassium pump. Following each procedure, the heart was freeze dried so that diffusion did not occur, thereby causing a change in the experiment. Using the Scanning Electron Microscopy (SEM), Rubidium will be measured in the cardiac tissue to determine the amount of Rubidium absorbed into the cells. Rubidium will be measured using the Energy-Dispersive X-Ray Spectroscopy system (EDS) associated with the SEM (JEOL JSM-6510LV). The heart is expected to beat for about one hour during which all necessary procedures regarding the use of the heart will take place. Rubidium is also expected to be taken up by the cells of the heart in positive slope with respect to time while the hearts continues to beat. When ouabain is introduced, the sodium/potassium pump activity will slow down. With ouabain, the level of rubidium uptake is expected to decrease while sodium is expected to accumulate inside the cell, causing the heart to stop beating.

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Apr 21st, 10:00 AM Apr 21st, 11:30 AM

Rubidium as Substitute for Potassium in Rat Cardiac Tissue

CSU Ballroom

A normal cell contains a sodium/potassium ATPase pump that functions to maintain a high intracellular level of potassium (K+) inside the cell and high levels of sodium (Na+) outside the cell. Since rubidium (Rb+) shares similar chemical properties with potassium and has a similar atomic radius it can replace potassium in all known processes (Olga Jilkina et al). We used rat hearts as a model to determine and quantify how rubidium will take over as potassium in the sodium/potassium pump. First we used the Langendorff system to infuse the aorta of the rat heart with a buffer solution containing Rubidium Chloride (RbCl) so that it circulates through the coronary arteries. The Langendorff apparatus provides a means to control pH, temperature and pressure of the solutions perfusing the heart. A sequel to this procedure is the addition of ouabain to the buffer solution, which will inhibit the sodium/potassium pump. Following each procedure, the heart was freeze dried so that diffusion did not occur, thereby causing a change in the experiment. Using the Scanning Electron Microscopy (SEM), Rubidium will be measured in the cardiac tissue to determine the amount of Rubidium absorbed into the cells. Rubidium will be measured using the Energy-Dispersive X-Ray Spectroscopy system (EDS) associated with the SEM (JEOL JSM-6510LV). The heart is expected to beat for about one hour during which all necessary procedures regarding the use of the heart will take place. Rubidium is also expected to be taken up by the cells of the heart in positive slope with respect to time while the hearts continues to beat. When ouabain is introduced, the sodium/potassium pump activity will slow down. With ouabain, the level of rubidium uptake is expected to decrease while sodium is expected to accumulate inside the cell, causing the heart to stop beating.

Recommended Citation

Asaolu, Bisola and Mai Vue. "Rubidium as Substitute for Potassium in Rat Cardiac Tissue." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
http://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/1