Event Title

The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)

Location

CSU Ballroom

Start Date

21-4-2014 10:00 AM

End Date

21-4-2014 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Steven Mercurio

Mentor's Email Address

steven.mercurio@mnsu.edu

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Acetaminophen is an OTC pain reliever that causes liver damage on overdose. Lower doses affect the kidneys, especially in male mice were the liver metabolite is toxic. Metformin is a medication that is used for dietary-induced diabetes and is similarly toxic to the liver and kidney, especially if compromised by other damage already. Lactic acidosis can further damage organs. The effects of childhood obesity on this medication have not been fully evaluated. The model of dietary-induced obese male mouse is comparable. The expected outcome of a high dose of acetaminophen with metformin will be liver and kidney damage as seen in the organ and the blood chemistry changes. The null hypothesis is that these changes are not observed. The mice will have free access to 11% fat diet to further fatten them over a four week period, then will be put into four groups of four and begin their experimental treatments for four weeks. The four trials will be no medication, acetaminophen, metformin, and a mixture of acetaminophen and metformin. Elevated levels of bilirubin or creatinine may indicate liver or kidney problems and can monitor changes in organ function. Mice will be euthanized using CO2. Serum samples will be taken for a HPLC assay, as well as blood and tissue samples. Blood bilirubin levels will be measured using direct spectrophotometry and a tnf-alpha assay will be done using lysed cells. Kidneys and liver will be examined for physical damage, and tissue pH will be tested. Bodies disposed of by EHS.

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Apr 21st, 10:00 AM Apr 21st, 11:30 AM

The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)

CSU Ballroom

Acetaminophen is an OTC pain reliever that causes liver damage on overdose. Lower doses affect the kidneys, especially in male mice were the liver metabolite is toxic. Metformin is a medication that is used for dietary-induced diabetes and is similarly toxic to the liver and kidney, especially if compromised by other damage already. Lactic acidosis can further damage organs. The effects of childhood obesity on this medication have not been fully evaluated. The model of dietary-induced obese male mouse is comparable. The expected outcome of a high dose of acetaminophen with metformin will be liver and kidney damage as seen in the organ and the blood chemistry changes. The null hypothesis is that these changes are not observed. The mice will have free access to 11% fat diet to further fatten them over a four week period, then will be put into four groups of four and begin their experimental treatments for four weeks. The four trials will be no medication, acetaminophen, metformin, and a mixture of acetaminophen and metformin. Elevated levels of bilirubin or creatinine may indicate liver or kidney problems and can monitor changes in organ function. Mice will be euthanized using CO2. Serum samples will be taken for a HPLC assay, as well as blood and tissue samples. Blood bilirubin levels will be measured using direct spectrophotometry and a tnf-alpha assay will be done using lysed cells. Kidneys and liver will be examined for physical damage, and tissue pH will be tested. Bodies disposed of by EHS.

Recommended Citation

Hofmann, Quinn; Yuko Nakamura; and Sara Sobota. "The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
http://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/15