Event Title

Combining Oxyrase® and Conditioned Medium to Improve the Resuscitation of Dormant Mycobacterium Avium Subsp. Paratuberculosis

Location

CSU Ballroom

Start Date

20-4-2015 10:00 AM

End Date

20-4-2015 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Timothy Secott

Mentor's Email Address

timothy.secott@mnsu.edu

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Johne’s disease is a chronic intestinal disease that affects ruminants around the globe. This disease, which is caused by the acid-fast bacillus Mycobacterium avium subsp. paratuberculosis (MAP), causes sever economic losses to the US dairy industry. One of the problems associated with this disease is the inability to detect subclinically diseased animals, those in which the organism is in a dormant state. The purpose of our experiment was to determine whether the inclusion of conditioned medium (CM; containing growth factors produced by MAP), Oxyrase (a biological reducing agent), or both would enhance the growth of MAP. MAP was incubated in medium containing serial dilutions of Oxyrase, CM, or both, and growth was monitored every 2-3 days by measuring optical density. The growth responses of MAP to these substances will be presented. The findings of this research are expected to improve the sensitivity of diagnostic culture for MAP, thus helping to reduce the effect of John's disease on dairy farming and maintain a safe supply of dairy products for consumers.

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Apr 20th, 10:00 AM Apr 20th, 11:30 AM

Combining Oxyrase® and Conditioned Medium to Improve the Resuscitation of Dormant Mycobacterium Avium Subsp. Paratuberculosis

CSU Ballroom

Johne’s disease is a chronic intestinal disease that affects ruminants around the globe. This disease, which is caused by the acid-fast bacillus Mycobacterium avium subsp. paratuberculosis (MAP), causes sever economic losses to the US dairy industry. One of the problems associated with this disease is the inability to detect subclinically diseased animals, those in which the organism is in a dormant state. The purpose of our experiment was to determine whether the inclusion of conditioned medium (CM; containing growth factors produced by MAP), Oxyrase (a biological reducing agent), or both would enhance the growth of MAP. MAP was incubated in medium containing serial dilutions of Oxyrase, CM, or both, and growth was monitored every 2-3 days by measuring optical density. The growth responses of MAP to these substances will be presented. The findings of this research are expected to improve the sensitivity of diagnostic culture for MAP, thus helping to reduce the effect of John's disease on dairy farming and maintain a safe supply of dairy products for consumers.

Recommended Citation

Ramana, Chandru. "Combining Oxyrase® and Conditioned Medium to Improve the Resuscitation of Dormant Mycobacterium Avium Subsp. Paratuberculosis." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
http://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/22