Event Title

The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans

Location

CSU Ballroom

Start Date

20-4-2015 10:00 AM

End Date

20-4-2015 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Email Address

james.rife@mnsu.edu

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Phytophthora sojae (P. sojae) is the second most destructive pathogen to soybeans; however, the mechanism of its pathogenesis is not well understood. Recent studies have shown that microRNA 393 (miR393) is up-regulated in response to P. sojae infection. Methyl jasmonate (MeJa) is produced by plants in response to wounding. To probe for a possible link between P. sojae infection and a response elicited by MeJa, soybean plants were treated to three conditions and then miR393 expression was monitored using Quantitative Polymerase Chain Reaction (Q-PCR). Treatment groups consisted of a control set of plants, P. sojae-infected plants, and plants treated with MeJa. Soybean roots were inoculated by scraping the soybean roots 1.0 cm from the root end and applying a small plug of P. sojae-infected agar. The MeJa group was treated by exposing the plants to evaporated MeJa in an enclosed container. 8 hours post-treatment, root tissues were harvested and miRNAs were isolated using an Ambion Plant RNA Isolation Aid in conjunction with a mirVana miRNA isolation kit. The quality and quantity of small RNAs was assessed by spectrophotometric analysis and by denaturing polyacrylamide electrophoresis. miRNAs were reverse transcribed into cDNA using a TaqMan microRNA Reverse Transcription System. Q-PCR, with FAM-labeled probes, and the ΔΔCt method were used to measure relative miR393 levels using U6 as an endogenous control.

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Apr 20th, 10:00 AM Apr 20th, 11:30 AM

The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans

CSU Ballroom

Phytophthora sojae (P. sojae) is the second most destructive pathogen to soybeans; however, the mechanism of its pathogenesis is not well understood. Recent studies have shown that microRNA 393 (miR393) is up-regulated in response to P. sojae infection. Methyl jasmonate (MeJa) is produced by plants in response to wounding. To probe for a possible link between P. sojae infection and a response elicited by MeJa, soybean plants were treated to three conditions and then miR393 expression was monitored using Quantitative Polymerase Chain Reaction (Q-PCR). Treatment groups consisted of a control set of plants, P. sojae-infected plants, and plants treated with MeJa. Soybean roots were inoculated by scraping the soybean roots 1.0 cm from the root end and applying a small plug of P. sojae-infected agar. The MeJa group was treated by exposing the plants to evaporated MeJa in an enclosed container. 8 hours post-treatment, root tissues were harvested and miRNAs were isolated using an Ambion Plant RNA Isolation Aid in conjunction with a mirVana miRNA isolation kit. The quality and quantity of small RNAs was assessed by spectrophotometric analysis and by denaturing polyacrylamide electrophoresis. miRNAs were reverse transcribed into cDNA using a TaqMan microRNA Reverse Transcription System. Q-PCR, with FAM-labeled probes, and the ΔΔCt method were used to measure relative miR393 levels using U6 as an endogenous control.

Recommended Citation

Oeltjenbruns, Andrew. "The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
http://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/43