Event Title

Amygdala Morphology and Neurogenesis in the Green Anole Lizard

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Rachel Cohen

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Steroid hormones and their derivatives play a major role in the reproductive system. One region in the brain that is involved in reproduction is the amygdala. We are examining the relationship between steroid hormones and neuron size, number and neurogenesis in the amygdala of the green anole lizard (Anolis carolinensis). Green anoles are exceptionally good models to examine the neural control of reproductive behaviors because they are seasonally breeding animals and exhibit unique behavioral and physiological differences in the breeding season compared to the non-breeding season. These behavioral differences are likely caused by seasonal changes in circulating steroid hormone levels. For our project, breeding green anole males were gonadectomized and a capsule containing testosterone, estradiol, dihydrotestosterone or left empty was inserted under the anole’s skin. The animals were injected with bromodeoxyuridine (BrdU; a new cell marker) for three days after the treatment. After one month, brains were collected, sectioned, and placed on slides. An immunohistochemistry for BrdU and Hu (neuronal marker) was conducted to examine the presence of new neurons in the amygdala. Alternate sections were Nissl stained and used to count cell number and measure soma size. We expect to see a decrease in neuron number, soma size, and neurogenesis in the animals treated with hormones compared to the animals treated with the blank capsule because we see this pattern in breeding season animals. This work will help provide more insight into the neural control of reproduction.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Amygdala Morphology and Neurogenesis in the Green Anole Lizard

CSU Ballroom

Steroid hormones and their derivatives play a major role in the reproductive system. One region in the brain that is involved in reproduction is the amygdala. We are examining the relationship between steroid hormones and neuron size, number and neurogenesis in the amygdala of the green anole lizard (Anolis carolinensis). Green anoles are exceptionally good models to examine the neural control of reproductive behaviors because they are seasonally breeding animals and exhibit unique behavioral and physiological differences in the breeding season compared to the non-breeding season. These behavioral differences are likely caused by seasonal changes in circulating steroid hormone levels. For our project, breeding green anole males were gonadectomized and a capsule containing testosterone, estradiol, dihydrotestosterone or left empty was inserted under the anole’s skin. The animals were injected with bromodeoxyuridine (BrdU; a new cell marker) for three days after the treatment. After one month, brains were collected, sectioned, and placed on slides. An immunohistochemistry for BrdU and Hu (neuronal marker) was conducted to examine the presence of new neurons in the amygdala. Alternate sections were Nissl stained and used to count cell number and measure soma size. We expect to see a decrease in neuron number, soma size, and neurogenesis in the animals treated with hormones compared to the animals treated with the blank capsule because we see this pattern in breeding season animals. This work will help provide more insight into the neural control of reproduction.

Recommended Citation

Roddick, Jadden; Nicholas Booker; and Abodalrahman Algamdy. "Amygdala Morphology and Neurogenesis in the Green Anole Lizard." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/15