Event Title

Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Auxin plays important roles in plant growth and development including stem elongation, phototropic and gravitropic responses, apical dominance, and lateral and adventitious root formation. A plant’s response to auxin involves Auxin/Indole-3-acetic acid (Aux/IAA) proteins, Auxin Response Factors (ARFs), Auxin Signaling F-Box Proteins (AFB1, AFB2 and AFB3) and components of the protein degradation pathway. Due to plants being immobile, environmental stress can be detrimental to their productivity. Plants have evolved defense mechanisms to cope with these environmental stressors. These defense mechanisms often impact the auxin signaling pathway. The goal of this project was to investigate the effect of environmental stress on expression of AFB-2, Auxin Signaling F-Box2. Four groups of Glycine max (soybean) plants were grown. While one group served as a control, the other groups were treated with abiotic stressors including drought, cold temperature, and high salinity. mRNAs were isolated using a mirVana miRNA Isolation kit (Life Technologies). cDNA copies of the isolated mRNAs were made using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). Finally, Quantitative Polymerase Chain Reaction was performed to determine the relative quantities of the cDNA. The stresses had no significant effect on the expression of AFB2 in the leaves. However, root samples showed a 50% decrease in expression of AFB2 in drought and cold treated plants, whereas the high salinity treatment doubled the expression of AFB2 compared to the control.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA

CSU Ballroom

Auxin plays important roles in plant growth and development including stem elongation, phototropic and gravitropic responses, apical dominance, and lateral and adventitious root formation. A plant’s response to auxin involves Auxin/Indole-3-acetic acid (Aux/IAA) proteins, Auxin Response Factors (ARFs), Auxin Signaling F-Box Proteins (AFB1, AFB2 and AFB3) and components of the protein degradation pathway. Due to plants being immobile, environmental stress can be detrimental to their productivity. Plants have evolved defense mechanisms to cope with these environmental stressors. These defense mechanisms often impact the auxin signaling pathway. The goal of this project was to investigate the effect of environmental stress on expression of AFB-2, Auxin Signaling F-Box2. Four groups of Glycine max (soybean) plants were grown. While one group served as a control, the other groups were treated with abiotic stressors including drought, cold temperature, and high salinity. mRNAs were isolated using a mirVana miRNA Isolation kit (Life Technologies). cDNA copies of the isolated mRNAs were made using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). Finally, Quantitative Polymerase Chain Reaction was performed to determine the relative quantities of the cDNA. The stresses had no significant effect on the expression of AFB2 in the leaves. However, root samples showed a 50% decrease in expression of AFB2 in drought and cold treated plants, whereas the high salinity treatment doubled the expression of AFB2 compared to the control.

Recommended Citation

Dusek, Dane. "Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/35