Event Title
Reactive Oxygen Species Effects on Lysozyme
Location
CSU Ballroom
Start Date
18-4-2016 10:00 AM
End Date
18-4-2016 11:30 AM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
John Thoemke
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Reactive oxygen species (ROS) target specific amino acids within protein structures, then can cause molecular changes that disrupt the structure and function of the protein. Previous work with the protein lysozyme reacting with singlet oxygen shows that the protein’s biological activity is impaired. Fluorescence spectroscopy is used to elucidate the structure changes near specific amino acid residues. The procedure involves irradiating lysozyme solutions with visible light, in the presence of the sensitizer methylene blue. This irradiation forms singlet oxygen that is then available to react with lysozyme. Samples of lysozyme are exposed to irradiation over varying time increments. These samples are then tested by UV and fluorescence spectroscopy to find out how the samples change due to varying irradiation exposure. Changes in the fluorescence spectra for lysozyme exposed to varying time increments will help determine the kinetics of the reaction of lysozyme with singlet oxygen. Better understanding of the rate kinetics associated with singlet oxygen reacting with lysozyme will allow for greater classification of the effects singlet oxygen has on the structure and functionality of lysozyme.
Reactive Oxygen Species Effects on Lysozyme
CSU Ballroom
Reactive oxygen species (ROS) target specific amino acids within protein structures, then can cause molecular changes that disrupt the structure and function of the protein. Previous work with the protein lysozyme reacting with singlet oxygen shows that the protein’s biological activity is impaired. Fluorescence spectroscopy is used to elucidate the structure changes near specific amino acid residues. The procedure involves irradiating lysozyme solutions with visible light, in the presence of the sensitizer methylene blue. This irradiation forms singlet oxygen that is then available to react with lysozyme. Samples of lysozyme are exposed to irradiation over varying time increments. These samples are then tested by UV and fluorescence spectroscopy to find out how the samples change due to varying irradiation exposure. Changes in the fluorescence spectra for lysozyme exposed to varying time increments will help determine the kinetics of the reaction of lysozyme with singlet oxygen. Better understanding of the rate kinetics associated with singlet oxygen reacting with lysozyme will allow for greater classification of the effects singlet oxygen has on the structure and functionality of lysozyme.
Recommended Citation
Rachuy, Jacob. "Reactive Oxygen Species Effects on Lysozyme." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
https://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/38
