Event Title

Reactive Oxygen Species Effects on Lysozyme

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

John Thoemke

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Reactive oxygen species (ROS) target specific amino acids within protein structures, then can cause molecular changes that disrupt the structure and function of the protein. Previous work with the protein lysozyme reacting with singlet oxygen shows that the protein’s biological activity is impaired. Fluorescence spectroscopy is used to elucidate the structure changes near specific amino acid residues. The procedure involves irradiating lysozyme solutions with visible light, in the presence of the sensitizer methylene blue. This irradiation forms singlet oxygen that is then available to react with lysozyme. Samples of lysozyme are exposed to irradiation over varying time increments. These samples are then tested by UV and fluorescence spectroscopy to find out how the samples change due to varying irradiation exposure. Changes in the fluorescence spectra for lysozyme exposed to varying time increments will help determine the kinetics of the reaction of lysozyme with singlet oxygen. Better understanding of the rate kinetics associated with singlet oxygen reacting with lysozyme will allow for greater classification of the effects singlet oxygen has on the structure and functionality of lysozyme.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Reactive Oxygen Species Effects on Lysozyme

CSU Ballroom

Reactive oxygen species (ROS) target specific amino acids within protein structures, then can cause molecular changes that disrupt the structure and function of the protein. Previous work with the protein lysozyme reacting with singlet oxygen shows that the protein’s biological activity is impaired. Fluorescence spectroscopy is used to elucidate the structure changes near specific amino acid residues. The procedure involves irradiating lysozyme solutions with visible light, in the presence of the sensitizer methylene blue. This irradiation forms singlet oxygen that is then available to react with lysozyme. Samples of lysozyme are exposed to irradiation over varying time increments. These samples are then tested by UV and fluorescence spectroscopy to find out how the samples change due to varying irradiation exposure. Changes in the fluorescence spectra for lysozyme exposed to varying time increments will help determine the kinetics of the reaction of lysozyme with singlet oxygen. Better understanding of the rate kinetics associated with singlet oxygen reacting with lysozyme will allow for greater classification of the effects singlet oxygen has on the structure and functionality of lysozyme.

Recommended Citation

Rachuy, Jacob. "Reactive Oxygen Species Effects on Lysozyme." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/38