Event Title

Morphological and Genetic Characteristics of Strigeidae Trematodes In Waterfowl from Lake Winnibogoshish, Minnesota

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Robert Sorensen

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Second Mentor's Name

Tyler Achatz

Second Mentor's Department

Biological Sciences

Second Mentor's College

Science, Engineering and Technology

Description

The purpose of the study was to identify and analyze morphological and genetic variation within cryptic Family Strigeidae populations in waterfowl from Lake Winnibigoshish, Minnesota to determine species identities and potential lineage variation. DNA was extracted using a ZR tissue miniprep kit. Polymerase Chain Reaction (PCR) was done using primer sets MPLAT, PLAT, and JB (Cox1) for mitochondrial DNA and DIG-1200R (28s) and ITS4- 5 (ITS) for ribosomal DNA. mtDNA has been shown to track lineages and discrete geographical populations, while rDNA has been shown diagnostic use for species identifications across geographical regions with minimal variations. The DNA was sequenced using ABI377 DNA sequencer and analyzed using basefinder and 4-peaks software. Phylogenies were developed using MEGA6 software. Morphological data was taken from permanent slides made in a prior study. The results showed mitochondrial genetic variation between worms taken from different waterfowl hosts and from the same host in some cases indicating distinct lineages from Lake Winnibigoshish waterfowl. 28s DNA was found to be identical between individuals of the same species, while there was variance between other cryptic strigeid species. This supports the presence of multiple cryptic species and lineages within waterfowl from Lake Winnibigoshish. With the many body form variations present, a limited number of structures were identifiable for metric comparison. However, of the structures found there did appear to be variation between strigeid species and within a strigeid species in different hosts. The genital cone and bulb, two cryptic diagnostic traits for genus level diagnosis, were identified and photographed for future reference.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Morphological and Genetic Characteristics of Strigeidae Trematodes In Waterfowl from Lake Winnibogoshish, Minnesota

CSU Ballroom

The purpose of the study was to identify and analyze morphological and genetic variation within cryptic Family Strigeidae populations in waterfowl from Lake Winnibigoshish, Minnesota to determine species identities and potential lineage variation. DNA was extracted using a ZR tissue miniprep kit. Polymerase Chain Reaction (PCR) was done using primer sets MPLAT, PLAT, and JB (Cox1) for mitochondrial DNA and DIG-1200R (28s) and ITS4- 5 (ITS) for ribosomal DNA. mtDNA has been shown to track lineages and discrete geographical populations, while rDNA has been shown diagnostic use for species identifications across geographical regions with minimal variations. The DNA was sequenced using ABI377 DNA sequencer and analyzed using basefinder and 4-peaks software. Phylogenies were developed using MEGA6 software. Morphological data was taken from permanent slides made in a prior study. The results showed mitochondrial genetic variation between worms taken from different waterfowl hosts and from the same host in some cases indicating distinct lineages from Lake Winnibigoshish waterfowl. 28s DNA was found to be identical between individuals of the same species, while there was variance between other cryptic strigeid species. This supports the presence of multiple cryptic species and lineages within waterfowl from Lake Winnibigoshish. With the many body form variations present, a limited number of structures were identifiable for metric comparison. However, of the structures found there did appear to be variation between strigeid species and within a strigeid species in different hosts. The genital cone and bulb, two cryptic diagnostic traits for genus level diagnosis, were identified and photographed for future reference.

Recommended Citation

Botros, Stephanie and Rachel Eiler. "Morphological and Genetic Characteristics of Strigeidae Trematodes In Waterfowl from Lake Winnibogoshish, Minnesota." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/7