Event Title

miR160 and 167 and their Target mRNAs

Location

CSU Ballroom

Start Date

11-4-2017 10:00 AM

End Date

11-4-2017 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Corn is the second most prevalent crop across the world. Use of nitrogen fertilizer is a major factor in corn yields. However, nitrogen efficiency in cereal crops is estimated to be at only 33%.

Understanding how corn responds to changes in nitrogen levels can lead to an environmentally responsible increase in crop yields. Earlier work in this lab demonstrated that nitrate levels affect miRNA 160 and 167 levels in corn roots. These miRNAs are thought to target the mRNAs of auxin response factors (ARF). One-week-old corn plants were transferred to a hydroponic vessel containing Hoaglund’s solution minus nitrate. Two days after nitrogen starvation half of the plants were kept in Hoaglund’s minus nitrogen, and half were transferred to a Hoaglund’s with nitrogen solution. Root tips and root elongation zones were harvested at 1 hour and 22 hours after the transfer and analyzed for miRNA 160 and 167 levels. Nitrate was found to decrease levels of miR160 and 167 in the root tips and increase in the levels of 160 and 167 in the root elongation zones. MiR160 and 167 target mRNAs for ARF 17 and 6 respectively. These mRNAs will be examined to see if changes in their levels correlate with the miRNA levels.

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Apr 11th, 10:00 AM Apr 11th, 11:30 AM

miR160 and 167 and their Target mRNAs

CSU Ballroom

Corn is the second most prevalent crop across the world. Use of nitrogen fertilizer is a major factor in corn yields. However, nitrogen efficiency in cereal crops is estimated to be at only 33%.

Understanding how corn responds to changes in nitrogen levels can lead to an environmentally responsible increase in crop yields. Earlier work in this lab demonstrated that nitrate levels affect miRNA 160 and 167 levels in corn roots. These miRNAs are thought to target the mRNAs of auxin response factors (ARF). One-week-old corn plants were transferred to a hydroponic vessel containing Hoaglund’s solution minus nitrate. Two days after nitrogen starvation half of the plants were kept in Hoaglund’s minus nitrogen, and half were transferred to a Hoaglund’s with nitrogen solution. Root tips and root elongation zones were harvested at 1 hour and 22 hours after the transfer and analyzed for miRNA 160 and 167 levels. Nitrate was found to decrease levels of miR160 and 167 in the root tips and increase in the levels of 160 and 167 in the root elongation zones. MiR160 and 167 target mRNAs for ARF 17 and 6 respectively. These mRNAs will be examined to see if changes in their levels correlate with the miRNA levels.

Recommended Citation

Connolly, Patrick. "miR160 and 167 and their Target mRNAs." Undergraduate Research Symposium, Mankato, MN, April 11, 2017.
http://cornerstone.lib.mnsu.edu/urs/2017/poster-session-A/30