FAM171B is a relatively uncharacterized protein that contains fourteen consecutive glutamine residues within its primary amino acid sequence; thus it is a polyglutamine (polyQ) protein. PolyQ proteins are interesting because mutations within the polyQ tract have been linked to a number of severe neurodegenerative diseases including Huntingtons Disease (HD). To date, there are minimal published data regarding FAM171B and its molecular function, such that both expression levels and intracellular localization have yet to be definitively determined. Using immunohistochemical and in situ hybridization analyses, our lab has recently found that FAM171B is expressed throughout the mouse brain. This current study attempts to build off this information to identify the intracellular localization of FAM171B in several different nervous system cell types including: a human glioblastoma cell line (U138), a human neuroblastoma cell line (SH-SY5Y), and mouse brain primary neurons. Using immunofluorescence, FAM171B-GFP fusion proteins, and live cell imaging (coupled with confocal microscopy) our data indicate that FAM171B has a predominantly vesicular/punctate staining within the cytoplasm of cells. Furthermore, colocalization studies using organelle specific markers shows that FAM171B localizes to both endosomes and cellular regions near the plasma membrane. Taken together, our data suggests that FAM171B may play a vesicle trafficking role within cells of the nervous system.
Date of Degree
Master of Science (MS)
Science, Engineering and Technology
Rajaguru, D. (2020). Intracellular localization of the novel polyQ protein FAM171B [Master’s thesis, Minnesota State University, Mankato]. Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato. https://cornerstone.lib.mnsu.edu/etds/1063/
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