Cloning of ACC Oxidase from Cucumber and Muskmelon

Location

CSU

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

James E. Rife

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

The conversion of l-aminocyclopropane carboxylic acid (ACC) into ethyiene is catalyzed by an enzyme known as ACC oxidase. Ethyiene is a plant hormone that regulates many processes in plants, including ripening of the Iruit. In this research, two different fruits with similar gene sequences, cucumber and muskmelon, were studied. The ACC oxidase gene in the muskmelon pulp, the cucumber pulp, and the cucumber leaves was extracted as mRNA, converted into cDNA, and amplified by RT-PCR. It was then cloned into an expression vector and transformed. This provided a readily available source of ACC oxidase so that the enzyme could be physically and kinetically characterized

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Cloning of ACC Oxidase from Cucumber and Muskmelon

CSU

The conversion of l-aminocyclopropane carboxylic acid (ACC) into ethyiene is catalyzed by an enzyme known as ACC oxidase. Ethyiene is a plant hormone that regulates many processes in plants, including ripening of the Iruit. In this research, two different fruits with similar gene sequences, cucumber and muskmelon, were studied. The ACC oxidase gene in the muskmelon pulp, the cucumber pulp, and the cucumber leaves was extracted as mRNA, converted into cDNA, and amplified by RT-PCR. It was then cloned into an expression vector and transformed. This provided a readily available source of ACC oxidase so that the enzyme could be physically and kinetically characterized