Event Title

Discovering the Structural Interactions Between Potential Anti-Cancer Agent NSC 652287 and Guanine

Presenter Information

Mark Thompson, Minnesota State University, Mankato

Location

CSU Ballroom

Start Date

21-4-2008 1:00 PM

End Date

21-4-2008 3:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

The focus of this research was to standardize the cross-links that form between guanines when DNA is exposed to 2, 5-bis (5-hydroxymethyl-2-thienyl) furan, NSC 652287. NSC 652287 is derived from a family of thiophenes that are found in plants and have widely been used in medicine for therapeutic uses such as antibiotics, antifungal and antiviral compounds. Many of the details about how NSC 652287 reacts with DNA are unknown. NSC 652287 was examined for its activity toward guanosine and deoxyguanosine. Reactions with calf-thymus DNA and sequence specific oligonucleotides were also explored. The methods used in this research were derived from previous work done on similar alkylating compounds including crotonaldehyde and diepoxybutane. All samples were analyzed using liquid chromatography-mass spectrometry (LC/MS), and think layer chromatography (TLC). Analysis of LC/MS has shown that NSC 652287 reacts with the calf-thymus DNA, and TLC has shown that NSC 652287 reacts with guanine.