Event Title

Quantifying the Immunoreactivity of Polyclonal IGG and IGY

Location

CSU Ballroom

Start Date

28-4-2009 10:00 AM

End Date

28-4-2009 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Marilyn Hart

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Actin, a filament found in the cytoplasm found in all eukaryotic cells, contributes to cell shape, cell mobility, and to the organization of certain tissues such as striated muscle. Actin is regulated by a variety of proteins, including actin capping protein (CP). CP is composed of two subunits, an alpha and a beta subunit. One goal of Dr. Hart's laboratory is to determine if the alpha subunits have similar or distinct functions in cells and tissues. As a first step towards accomplishing this goal, we will determine the location of the alpha subunits in cells/tissues using antibodies specific for each alpha isoform. The goal of our research was to characterize recently generated chicken and rabbit anti-Dl and 2 IgG and IgY antibodies, quantifying their immonoreactivity. Murine hearts were removed, flash frozen, and the tissue solubilized. The proteins were separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred to Nitrocellulose (NC) for subsequent Western Blot analysis. The immobilized proteins were allowed to react with dilutions of the antibodies and visualized with a secondary antibody labeled with alkaline phosphatase. The reactive titers of both the rabbit anti-D 1 IgG and the chicken anti 2 IgG and IgY antibodies were determined, providing an initial characterization of the newly generated antibodies and suggesting an approximate working dilution for subsequent studies.

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Apr 28th, 10:00 AM Apr 28th, 12:00 PM

Quantifying the Immunoreactivity of Polyclonal IGG and IGY

CSU Ballroom

Actin, a filament found in the cytoplasm found in all eukaryotic cells, contributes to cell shape, cell mobility, and to the organization of certain tissues such as striated muscle. Actin is regulated by a variety of proteins, including actin capping protein (CP). CP is composed of two subunits, an alpha and a beta subunit. One goal of Dr. Hart's laboratory is to determine if the alpha subunits have similar or distinct functions in cells and tissues. As a first step towards accomplishing this goal, we will determine the location of the alpha subunits in cells/tissues using antibodies specific for each alpha isoform. The goal of our research was to characterize recently generated chicken and rabbit anti-Dl and 2 IgG and IgY antibodies, quantifying their immonoreactivity. Murine hearts were removed, flash frozen, and the tissue solubilized. The proteins were separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred to Nitrocellulose (NC) for subsequent Western Blot analysis. The immobilized proteins were allowed to react with dilutions of the antibodies and visualized with a secondary antibody labeled with alkaline phosphatase. The reactive titers of both the rabbit anti-D 1 IgG and the chicken anti 2 IgG and IgY antibodies were determined, providing an initial characterization of the newly generated antibodies and suggesting an approximate working dilution for subsequent studies.

Recommended Citation

Cheritu, Tizazu and Ben Weingartz. "Quantifying the Immunoreactivity of Polyclonal IGG and IGY." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/26