Relationship between APOBEC3A and Herpes Simplex Virus 1 UL39

Location

CSU Ballroom

Start Date

12-4-2022 2:00 PM

End Date

12-4-2022 3:30 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Allison Land

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

The protein APOBEC3A (A3A) is an enzyme that belongs to a human family of DNA-cytosine deaminases which act as an immune defense against multiple kinds of viruses. These types of enzymes achieve deactivation of viruses by deaminating Cytosine (C) to produce Uracil (U) in single stranded DNA. Understanding the protein-protein interactions of A3A with different viral proteins is of great interest since it gives rise to the opportunity of manipulating these interactions for the development of new treatments. This study aims to visualize the interaction between A3A and the viral protein that is produced by Herpes Simplex Virus 1 (HSV-1) gene UL39 using confocal microscopy. The gene UL39 is of interest since it codes for the large subunit of ribonucleotide reductase, an enzyme responsible for converting ribonucleotides to deoxyribonucleotides, an essential reaction for DNA synthesis. We expect that A3A will interact with the protein coded by UL39 since it has been previously shown that it counteracts the antiviral effects of A3A by eliminating the enzyme from the nucleus, where viral replication occurs. The visualization of A3A and UL39 can give us an insight into the strength and colocalization of this protein-protein interaction. Currently, effective therapies for the treatment of HSV-1 infections are limited, and infections caused by this virus can be life threatening to infected neonates or immunocompromised individuals. This study aims to provide evidence that can be used for further studies for the development of new HSV-1 treatments.

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Apr 12th, 2:00 PM Apr 12th, 3:30 PM

Relationship between APOBEC3A and Herpes Simplex Virus 1 UL39

CSU Ballroom

The protein APOBEC3A (A3A) is an enzyme that belongs to a human family of DNA-cytosine deaminases which act as an immune defense against multiple kinds of viruses. These types of enzymes achieve deactivation of viruses by deaminating Cytosine (C) to produce Uracil (U) in single stranded DNA. Understanding the protein-protein interactions of A3A with different viral proteins is of great interest since it gives rise to the opportunity of manipulating these interactions for the development of new treatments. This study aims to visualize the interaction between A3A and the viral protein that is produced by Herpes Simplex Virus 1 (HSV-1) gene UL39 using confocal microscopy. The gene UL39 is of interest since it codes for the large subunit of ribonucleotide reductase, an enzyme responsible for converting ribonucleotides to deoxyribonucleotides, an essential reaction for DNA synthesis. We expect that A3A will interact with the protein coded by UL39 since it has been previously shown that it counteracts the antiviral effects of A3A by eliminating the enzyme from the nucleus, where viral replication occurs. The visualization of A3A and UL39 can give us an insight into the strength and colocalization of this protein-protein interaction. Currently, effective therapies for the treatment of HSV-1 infections are limited, and infections caused by this virus can be life threatening to infected neonates or immunocompromised individuals. This study aims to provide evidence that can be used for further studies for the development of new HSV-1 treatments.

Recommended Citation

Quevedo, Maria Rodriguez. "Relationship between APOBEC3A and Herpes Simplex Virus 1 UL39." Undergraduate Research Symposium, Mankato, MN, April 12, 2022.
https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/1