Event Title

Intracellular Localization of FAM171B-GFP Using Different Fixing Techniques

Location

CSU Ballroom

Start Date

16-4-2013 10:00 AM

End Date

16-4-2013 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Geoffrey Goellner

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

The main focus of our research is on the protein FAM171B. This protein was recently coded in a huge effort by the human genome project that coded the entire human genome. The little amount of information that is actually known about this protein is what makes it so interesting. It has a repeating protein sequence known as a polyQ region similar to neurodegenerative diseases such as Huntington’s. We have no information on the cellular presence of the protein, but finding the intracellular location of an unknown protein will help describe the function it has within the cell.

Using the vector FAM171B-GFP we transferred it into cultured cells by using a standard cell biological technique called transfection. We used three different fixing agents’ paraformaldehyde, methanol, and acetone to see where the fusion protein localized within the cell. From past experiments in our laboratory we have found a cytoplasmic vesicular staining pattern after fixing with methanol. We are comparing the results of the different fixing agents to see where the protein localizes within the cell, but due to inconclusive results thus far more testing is needed. Using the future results we will be able to conclude with certainty where the protein is expressed inside the cell.

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Apr 16th, 10:00 AM Apr 16th, 12:00 PM

Intracellular Localization of FAM171B-GFP Using Different Fixing Techniques

CSU Ballroom

The main focus of our research is on the protein FAM171B. This protein was recently coded in a huge effort by the human genome project that coded the entire human genome. The little amount of information that is actually known about this protein is what makes it so interesting. It has a repeating protein sequence known as a polyQ region similar to neurodegenerative diseases such as Huntington’s. We have no information on the cellular presence of the protein, but finding the intracellular location of an unknown protein will help describe the function it has within the cell.

Using the vector FAM171B-GFP we transferred it into cultured cells by using a standard cell biological technique called transfection. We used three different fixing agents’ paraformaldehyde, methanol, and acetone to see where the fusion protein localized within the cell. From past experiments in our laboratory we have found a cytoplasmic vesicular staining pattern after fixing with methanol. We are comparing the results of the different fixing agents to see where the protein localizes within the cell, but due to inconclusive results thus far more testing is needed. Using the future results we will be able to conclude with certainty where the protein is expressed inside the cell.

Recommended Citation

Gilbert, Brian and Steven Gilbert. "Intracellular Localization of FAM171B-GFP Using Different Fixing Techniques." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
http://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/20