Event Title

Seasonal Effects on Kisspeptin Concentration in the Green Anole Lizard, Anolis carolinensis

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Rachel Cohen

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Gonadal steroid hormones are responsible for reproductive behaviors; disruption in production of these hormones is also linked to fertility issues. The hypothalamic-pituitary- gonadal (HPG) axis controls the production of sex steroid hormones, testosterone and estradiol. A peptide, kisspeptin, stimulates this axis by acting on neurons in the hypothalamus. The green anole lizard, Anolis carolinensis, is a seasonally breeding animal that shows drastic changes in behavior and physiology between the breeding and non- breeding seasons. One such change is a large increase in testosterone levels in the breeding season compared to the non-breeding season. These fluctuations in testosterone concentration in green anoles allows for a great opportunity to study the HPG axis. In the current study, we used brain tissue from breeding and non-breeding season green anoles to perform western blot analysis on kisspeptin concentration. Due to the increase in testosterone in the breeding season, we hypothesized that an increase in kisspeptin concentrations will be observed in breeding season compared to the non-breeding season lizards. These results would suggest that kisspeptin does indeed play a role in stimulating the HPG axis and that kisspeptin could potentially be used as a treatment for infertility.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Seasonal Effects on Kisspeptin Concentration in the Green Anole Lizard, Anolis carolinensis

CSU Ballroom

Gonadal steroid hormones are responsible for reproductive behaviors; disruption in production of these hormones is also linked to fertility issues. The hypothalamic-pituitary- gonadal (HPG) axis controls the production of sex steroid hormones, testosterone and estradiol. A peptide, kisspeptin, stimulates this axis by acting on neurons in the hypothalamus. The green anole lizard, Anolis carolinensis, is a seasonally breeding animal that shows drastic changes in behavior and physiology between the breeding and non- breeding seasons. One such change is a large increase in testosterone levels in the breeding season compared to the non-breeding season. These fluctuations in testosterone concentration in green anoles allows for a great opportunity to study the HPG axis. In the current study, we used brain tissue from breeding and non-breeding season green anoles to perform western blot analysis on kisspeptin concentration. Due to the increase in testosterone in the breeding season, we hypothesized that an increase in kisspeptin concentrations will be observed in breeding season compared to the non-breeding season lizards. These results would suggest that kisspeptin does indeed play a role in stimulating the HPG axis and that kisspeptin could potentially be used as a treatment for infertility.

Recommended Citation

Booker, Nicholas and Hyejoo Kang. "Seasonal Effects on Kisspeptin Concentration in the Green Anole Lizard, Anolis carolinensis." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/22