1st Student's Major
Chemistry and Geology
1st Student's College
Science, Engineering and Technology
Students' Professional Biography
Samee Ranginwala joined Minnesota State University, Mankato in the Fall of 2006. He is pursuing a BS in Biochemistry and Human Biology. Samee began his research in the spring of 2009 under the guidance of Dr. James Rife and Dr. Terry Salerno the Department of Chemistry and Geology. His focus was mainly on the biochemical basis of plant defence mechanisms. Samee presented his research at the 11th Annual Undergraduate Research Conference at Minnesota State University. Upon graduating, Samee plans to pursue medicine.
Mentor's Name
James Rife
Mentor's Email Address
james.rife@mnsu.edu
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Abstract
Fatty Acid 9/13-Hydroperoxide Lyase (9/13-HPL) in the cucumber plant is an enzyme that cleaves either 9- or 13-hydroperoxides of polyunsaturated fatty acids to form volatile C9 or C6 aldehydes respectively. Since these aldehydes may play a role in the plant’s defenses against pathogens (K. Matsui, et. al. Phytochemistry 67 (2006) 649-657), the enzyme is stress induced during vulnerable times such as when the plant is injured. In order to better understand how this enzyme is induced, we have developed a Real Time Polymerase Chain Reaction method to quantitate levels of 9/13-HPL mRNA. In initial experiments, the effect of mechanical wounding of cucumber cotyledons on the transcription levels of the 9/13-HPL gene in wounded tissue and unwounded leaves was examined. The RNeasy Plant Minikit from Qiagen was used to isolate mRNA from the plant tissue and SYBR Green was used as the detection system for Real Time PCR.
Recommended Citation
Ranginwala, Samee
(2009)
"Expression of 9/13 Hydroperoxide Lyase in Cucumber Leaves,"
Journal of Undergraduate Research at Minnesota State University, Mankato: Vol. 9, Article 14.
DOI: https://doi.org/10.56816/2378-6949.1067
Available at:
https://cornerstone.lib.mnsu.edu/jur/vol9/iss1/14
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