Event Title

Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis

Location

CSU Ballroom

Start Date

21-4-2014 10:00 AM

End Date

21-4-2014 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Email Address

james.rife@mnsu.edu

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Soybeans are an important crop that not only provide nutrition for humans and livestock, but are also a potential source of biofuels. However, it is estimated that 70% of yields are lost due to unfavorable environments. Studying the defensive mechanisms of soybeans may help increase yields. Soybeans produce enzymes called lipoxygenases, which are involved in the synthesis of molecules, such as methyl jasmonate that respond to stress. These proteins were the focus of our study. In this project, a two-dimensional gel electrophoresis method was developed to monitor changes in the expression of soybean leaf proteins in response to stress factors. This method allowed proteins to be separated based on their isoelectric point, or the pH at which they have no net charge, and their molecular weight. Edamame “Be Sweet 292” beans were grown to the trifoliate stage and treated at about three weeks post-emergence. The plants were treated either by wounding with forceps, exposure to methyl jasmonate, or both. Control plants received no treatment. The leaf tissue was harvested 24 hours after the initiation of treatment and frozen with liquid nitrogen. The protein extraction procedure was modified from Sarma et al. (2008 Analytical Biochemistry, 379, 192-195). Proteins were separated by isoelectric focusing on BioRad IPG strips with a pH range of 3-10. They were then separated by SDS electrophoresis using Criterion XT 12% Bis-Tris gels and stained with gel code blue. This project will focus on the visualization of high molecular weight proteins such as lipoxygenase.

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Apr 21st, 10:00 AM Apr 21st, 11:30 AM

Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis

CSU Ballroom

Soybeans are an important crop that not only provide nutrition for humans and livestock, but are also a potential source of biofuels. However, it is estimated that 70% of yields are lost due to unfavorable environments. Studying the defensive mechanisms of soybeans may help increase yields. Soybeans produce enzymes called lipoxygenases, which are involved in the synthesis of molecules, such as methyl jasmonate that respond to stress. These proteins were the focus of our study. In this project, a two-dimensional gel electrophoresis method was developed to monitor changes in the expression of soybean leaf proteins in response to stress factors. This method allowed proteins to be separated based on their isoelectric point, or the pH at which they have no net charge, and their molecular weight. Edamame “Be Sweet 292” beans were grown to the trifoliate stage and treated at about three weeks post-emergence. The plants were treated either by wounding with forceps, exposure to methyl jasmonate, or both. Control plants received no treatment. The leaf tissue was harvested 24 hours after the initiation of treatment and frozen with liquid nitrogen. The protein extraction procedure was modified from Sarma et al. (2008 Analytical Biochemistry, 379, 192-195). Proteins were separated by isoelectric focusing on BioRad IPG strips with a pH range of 3-10. They were then separated by SDS electrophoresis using Criterion XT 12% Bis-Tris gels and stained with gel code blue. This project will focus on the visualization of high molecular weight proteins such as lipoxygenase.

Recommended Citation

Phelps, Emma. "Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
http://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/40