Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis
Location
CSU Ballroom
Start Date
21-4-2014 10:00 AM
End Date
21-4-2014 11:30 AM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Email Address
james.rife@mnsu.edu
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Soybeans are an important crop that not only provide nutrition for humans and livestock, but are also a potential source of biofuels. However, it is estimated that 70% of yields are lost due to unfavorable environments. Studying the defensive mechanisms of soybeans may help increase yields. Soybeans produce enzymes called lipoxygenases, which are involved in the synthesis of molecules, such as methyl jasmonate that respond to stress. These proteins were the focus of our study. In this project, a two-dimensional gel electrophoresis method was developed to monitor changes in the expression of soybean leaf proteins in response to stress factors. This method allowed proteins to be separated based on their isoelectric point, or the pH at which they have no net charge, and their molecular weight. Edamame “Be Sweet 292” beans were grown to the trifoliate stage and treated at about three weeks post-emergence. The plants were treated either by wounding with forceps, exposure to methyl jasmonate, or both. Control plants received no treatment. The leaf tissue was harvested 24 hours after the initiation of treatment and frozen with liquid nitrogen. The protein extraction procedure was modified from Sarma et al. (2008 Analytical Biochemistry, 379, 192-195). Proteins were separated by isoelectric focusing on BioRad IPG strips with a pH range of 3-10. They were then separated by SDS electrophoresis using Criterion XT 12% Bis-Tris gels and stained with gel code blue. This project will focus on the visualization of high molecular weight proteins such as lipoxygenase.
Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis
CSU Ballroom
Soybeans are an important crop that not only provide nutrition for humans and livestock, but are also a potential source of biofuels. However, it is estimated that 70% of yields are lost due to unfavorable environments. Studying the defensive mechanisms of soybeans may help increase yields. Soybeans produce enzymes called lipoxygenases, which are involved in the synthesis of molecules, such as methyl jasmonate that respond to stress. These proteins were the focus of our study. In this project, a two-dimensional gel electrophoresis method was developed to monitor changes in the expression of soybean leaf proteins in response to stress factors. This method allowed proteins to be separated based on their isoelectric point, or the pH at which they have no net charge, and their molecular weight. Edamame “Be Sweet 292” beans were grown to the trifoliate stage and treated at about three weeks post-emergence. The plants were treated either by wounding with forceps, exposure to methyl jasmonate, or both. Control plants received no treatment. The leaf tissue was harvested 24 hours after the initiation of treatment and frozen with liquid nitrogen. The protein extraction procedure was modified from Sarma et al. (2008 Analytical Biochemistry, 379, 192-195). Proteins were separated by isoelectric focusing on BioRad IPG strips with a pH range of 3-10. They were then separated by SDS electrophoresis using Criterion XT 12% Bis-Tris gels and stained with gel code blue. This project will focus on the visualization of high molecular weight proteins such as lipoxygenase.
Recommended Citation
Phelps, Emma. "Comparison of Protein Expression in Soy Bean by Two-Dimensional Electrophoresis." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
https://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/40
