Event Title

Vascular Regrowth after Partial Hepatectomy in Rats

Location

CSU Ballroom

Start Date

20-4-2015 10:00 AM

End Date

20-4-2015 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Michael Bentley

Mentor's Email Address

michael.bentley@mnsu.edu

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Cells in liver tissue have a remarkable capability for regeneration. However, the changes in vasculature during the regeneration process are poorly understood. In this study, the vasculature of liver was investigated in rats that had undergone partial hepatectomy. Livers were perfused with 2.5% glutaraldehyde in 0.1M phosphate buffer (pH 7.2). Pieces of tissue were immersed in 1% osmium tetroxide for one hour, dehydrated in grade acetone solution and critical point dried for observation using scanning electron microscopy. The appearance of the inner walls of blood vessels appeared to be related to regeneration time. These observations indicate that vasculature changes during liver regeneration could be important in disease conditions such as hepatitis and cirrhosis.

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Apr 20th, 10:00 AM Apr 20th, 11:30 AM

Vascular Regrowth after Partial Hepatectomy in Rats

CSU Ballroom

Cells in liver tissue have a remarkable capability for regeneration. However, the changes in vasculature during the regeneration process are poorly understood. In this study, the vasculature of liver was investigated in rats that had undergone partial hepatectomy. Livers were perfused with 2.5% glutaraldehyde in 0.1M phosphate buffer (pH 7.2). Pieces of tissue were immersed in 1% osmium tetroxide for one hour, dehydrated in grade acetone solution and critical point dried for observation using scanning electron microscopy. The appearance of the inner walls of blood vessels appeared to be related to regeneration time. These observations indicate that vasculature changes during liver regeneration could be important in disease conditions such as hepatitis and cirrhosis.

Recommended Citation

Paumen, Andrea; Rachel Thompson; and Rachel Ledding. "Vascular Regrowth after Partial Hepatectomy in Rats." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
http://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/12