Vascular Regrowth after Partial Hepatectomy in Rats
Location
CSU Ballroom
Start Date
20-4-2015 10:00 AM
End Date
20-4-2015 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Email Address
michael.bentley@mnsu.edu
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Cells in liver tissue have a remarkable capability for regeneration. However, the changes in vasculature during the regeneration process are poorly understood. In this study, the vasculature of liver was investigated in rats that had undergone partial hepatectomy. Livers were perfused with 2.5% glutaraldehyde in 0.1M phosphate buffer (pH 7.2). Pieces of tissue were immersed in 1% osmium tetroxide for one hour, dehydrated in grade acetone solution and critical point dried for observation using scanning electron microscopy. The appearance of the inner walls of blood vessels appeared to be related to regeneration time. These observations indicate that vasculature changes during liver regeneration could be important in disease conditions such as hepatitis and cirrhosis.
Vascular Regrowth after Partial Hepatectomy in Rats
CSU Ballroom
Cells in liver tissue have a remarkable capability for regeneration. However, the changes in vasculature during the regeneration process are poorly understood. In this study, the vasculature of liver was investigated in rats that had undergone partial hepatectomy. Livers were perfused with 2.5% glutaraldehyde in 0.1M phosphate buffer (pH 7.2). Pieces of tissue were immersed in 1% osmium tetroxide for one hour, dehydrated in grade acetone solution and critical point dried for observation using scanning electron microscopy. The appearance of the inner walls of blood vessels appeared to be related to regeneration time. These observations indicate that vasculature changes during liver regeneration could be important in disease conditions such as hepatitis and cirrhosis.
Recommended Citation
Paumen, Andrea; Rachel Thompson; and Rachel Ledding. "Vascular Regrowth after Partial Hepatectomy in Rats." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
https://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/12
