Event Title

Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels

Location

CSU Ballroom

Start Date

18-4-2016 10:00 AM

End Date

18-4-2016 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Michael Bentley

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Osteoporosis is common in post-menopausal women and in later years, of men as hormone levels decline. Calcium and phosphorus homeostasis is a complex processes involving many different components. More than 99% of total body calcium and phosphorus is stored in bone in the form of phosphate and hydroxide salts. Part of this research aimed to look at bone density, calcium levels, and phosphorus levels in both castrated and uncastrated (control) male C57/BL6 mice. The control group consisted of six non-castrated male mice. The experimental group consisted of six mice, which had been anesthetized with isoflurane and castrated using aseptic surgical technique. Three to four weeks after surgery, the mice were euthanized and the long bones were harvested, and weighed for bone density measurement. Calcium and phosphorus levels were measured using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS). The results of this research will provide information about the effect of low levels of testosterone on bone mineralization.

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Apr 18th, 10:00 AM Apr 18th, 11:30 AM

Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels

CSU Ballroom

Osteoporosis is common in post-menopausal women and in later years, of men as hormone levels decline. Calcium and phosphorus homeostasis is a complex processes involving many different components. More than 99% of total body calcium and phosphorus is stored in bone in the form of phosphate and hydroxide salts. Part of this research aimed to look at bone density, calcium levels, and phosphorus levels in both castrated and uncastrated (control) male C57/BL6 mice. The control group consisted of six non-castrated male mice. The experimental group consisted of six mice, which had been anesthetized with isoflurane and castrated using aseptic surgical technique. Three to four weeks after surgery, the mice were euthanized and the long bones were harvested, and weighed for bone density measurement. Calcium and phosphorus levels were measured using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS). The results of this research will provide information about the effect of low levels of testosterone on bone mineralization.

Recommended Citation

Rubitschung, Katie and Casey Hovey. "Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
http://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/24