Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels
Location
CSU Ballroom
Start Date
18-4-2016 10:00 AM
End Date
18-4-2016 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Osteoporosis is common in post-menopausal women and in later years, of men as hormone levels decline. Calcium and phosphorus homeostasis is a complex processes involving many different components. More than 99% of total body calcium and phosphorus is stored in bone in the form of phosphate and hydroxide salts. Part of this research aimed to look at bone density, calcium levels, and phosphorus levels in both castrated and uncastrated (control) male C57/BL6 mice. The control group consisted of six non-castrated male mice. The experimental group consisted of six mice, which had been anesthetized with isoflurane and castrated using aseptic surgical technique. Three to four weeks after surgery, the mice were euthanized and the long bones were harvested, and weighed for bone density measurement. Calcium and phosphorus levels were measured using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS). The results of this research will provide information about the effect of low levels of testosterone on bone mineralization.
Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels
CSU Ballroom
Osteoporosis is common in post-menopausal women and in later years, of men as hormone levels decline. Calcium and phosphorus homeostasis is a complex processes involving many different components. More than 99% of total body calcium and phosphorus is stored in bone in the form of phosphate and hydroxide salts. Part of this research aimed to look at bone density, calcium levels, and phosphorus levels in both castrated and uncastrated (control) male C57/BL6 mice. The control group consisted of six non-castrated male mice. The experimental group consisted of six mice, which had been anesthetized with isoflurane and castrated using aseptic surgical technique. Three to four weeks after surgery, the mice were euthanized and the long bones were harvested, and weighed for bone density measurement. Calcium and phosphorus levels were measured using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS). The results of this research will provide information about the effect of low levels of testosterone on bone mineralization.
Recommended Citation
Rubitschung, Katie and Casey Hovey. "Structure and Anatomy of Bone from Male C57/BL6 Mice with Reduced Testosterone Levels." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
https://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/24
