The Effect of Intra-Muscular Injections of Alpha-tocopherol on the Activity of Phospho- Fructokinase in the Slow- and Fast-Twitch Skeletal Muscles of Metabolic Stress-Induced and Malnourished Rats
Document Type
Article
Publication Date
12-2012
Abstract
It was been hypothesized that critical illness will change the activity of phospho-fructokinase (PFK) and that intramuscular (i.m) injections of the antioxidant α-tocopherol would protect this rate-limiting enzyme of glycolysis in the slow-twitch soleus and in the fast-twitch extensor digitorum longus (EDL) muscles of metabolically-stressed and malnourished rats. Twenty eight male Wistar rats were divided into four groups: controls (CNs), zymosan-injected (ZY), α-tocopherol-injected (ZYE), and pair-fed (PF). Rats were fed Osmolite-HN, and were sacrificed on day three. PFK activity was measured in EDL and soleus muscles using a fluorometric assay, and was found to be significantly lower in both the soleus and EDL of ZY rats. The PFK activity in the EDL of ZYE rats was comparable to the PFK of PF rats and was significantly higher than ZY rats on day three. Glycolysis in the slow- and fast-twitch muscle of ZY rats is adversely affected, and three days of i.m injections of α-tocopherol significantly (p
Department
Family Consumer Science, Chemistry and Geology
Online ISSN
2251-3140
Publication Title
GSTF Journal of BioSciences
Recommended Citation
Bissonnette, D. J., Hadley, M., Ying He, & Kubow, S. (2012). The Effect of Intra-Muscular Injections of Alpha-tocopherol on the Activity of Phospho-Fructokinase in the Slow- and Fast-Twitch Skeletal Muscles of Metabolic Stress-Induced and Malnourished Rats. GSTF Journal of BioSciences, 2(1), 12–24.
