Evidence of Oxidative Stress in Temporomandibular Disorders: A Pilot Study

Document Type

Article

Publication Date

10-2011

Abstract

Oxidative stress is involved in the pathogenesis of many conditions and is caused by free radicals in concentrations that overwhelm the natural scavenging mechanisms and cause pain and inflammation. This investigation sought to deter-mine whether pain from temporomandibular dis-orders was associated with increased oxidative stress as measured by biomarkers in saliva and serum. Both salivary and serum levels of the oxidative stress biomarkers including 8-hydroxydeoxyguanosine, malondialdehyde and total antioxidant status were compared in patients with mild and severe TMJD pain and with healthy controls. These biomarkers were determined spectrophotometrically in saliva and serum from 10 high TMJD pain patients, 10 lowTMJD pain patients, and 10 healthy control subjects from National Institute of Dental Research’s TMJ Implant Registry and Repository. Linear and logistic regression analyses were used to evaluate the association between each biomarker and TMJD pain.The mean levels of log 8-hydroxydeoxyguanosine(salivaP<0Æ0001, serumP=0Æ0008), malondialdehyde (salivaP=0Æ002, serumP=0Æ004) and totalantioxidant status (salivaP=0Æ005; serumP=0Æ001)achieved statistically significant differences between groups. In linear regression analysis, both salivary and serum levels of each biomarker were associated with TMJD pain. In a multivariable analysis, again,both salivary levels and serum levels were also different between groups. Salivary levels of oxidative stress ratios of 8-hydroxydeoxyguanosine, malondialdehyde and total antioxidant status were significantly different between patients with TMJD pain and controls and was comparable to that in serum. These biomarkers hold promise as a potential diagnostic and therapeutic strategy.

Department

Chemistry and Geology

Online ISSN

1365-2842

Publication Title

Journal of Oral Rehabilitation

DOI

10.1111/j.1365-2842.2011.02216.x

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