Title

Altered Vitamin A Homeostasis and Increased Size and Adiposity in the rdh1-null Mouse

Document Type

Article

Publication Date

4-30-2007

Abstract

Rat RoDH performs efficiently (Vm/Km) in a pathway of all-íraíw-retinoic acid biosynthesis in cells and recognizes the physiological form of vitamin A, i.e., retinol bound with cellular retinol binding-protein, type I. Here we report that mouse embryo (e7.5 to e18.5) and liver (e12.5 to P2M) display inversely related mRNA expression of an Rodh ortholog, rdhl, and a major retinoic acid catabolic enzyme, cyp26a1, suggesting coordinate modulation of retinoic acid homeostasis. Rdhl inactivation by homologous recombination produces mice with decreased liver cyp26a1 mRNA and protein and increased liver and kidney retinoid stores, when fed vitamin A-restricted diets. Thus, null mice autocompensate by down-regulating cyp26a1 and sparing retinoids, indicating that rdhl metabolizes retinoids in vivo. Surprisingly, rdhl-null mice grow longer than wild type, with increased weight and adiposity, when restricted in vitamin A. Liver, kidney, and multiple fat pads increase in weight. Some differences reflect the larger sizes of rdhl-null mice, but mesentery, femoral, and inguinal fat pads grow disproportionately larger. These data reveal an unexpected contribution of Rdh1 to size and adiposity and provide the first genetic evidence of a candidate retinol dehydrogenase affecting either vitamin A-re-lated homeostasis physiologically or vitamin A-related gene expression or biological function in vivo.

Department

Chemistry and Geology

Print ISSN

0892-6638

Publication Title

The FASEB Journal

DOI

10.1096/fj.06-7964com

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