This laboratory study examined the effects of acetaminophen overdose in normal fed and high-fat fed female mice. Forty female mice were placed on normal and high-fat diets at 4 weeks old. When the mice were significantly different in weight (between 6 and 9 months old), half the mice were dosed with acetaminophen. These mice were daily given an overdose of acetaminophen for 14 days. The dose used was 300 mg/kg mouse (LD50 = 338 mg/kg). The control group was given 10 µl water/g mouse. Levels of serum glutamate dehydrogenase (GDH) were measured to indicate liver damage. GDH is released from liver mitochondria indicating mitochondrial damage and toxicity. Based on a two-way ANOVA, the mean GDH levels were significantly higher in the high-fat diet mice groups (p < 0.001). This suggests damage to liver mitochondria due to high-fat diet alone. Liver weights of all female mice groups were documented and statistically analyzed and showed significant difference between diet groups (p < 0.001) and diet*treatment groups (p = 0.026). Visible fat content in all livers was also analyzed and showed significant difference between diet groups (p < 0.001) and treatment (acetaminophen or control) groups (p = 0.001).
Date of Degree
Master of Science (MS)
Science, Engineering and Technology
Behmer, Elizabeth Ruth, "High Fat Diet Increased Serum Glutamate Dehydrogenase more than Chronic Acetaminophen Dosing in Female Mice" (2014). All Theses, Dissertations, and Other Capstone Projects. 370.
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