Abstract

Congestive Heart failure is amongst the leading causes of death in the United States. It is treated through a variety of different therapeutic targets including the inhibition of sodium potassium pumps. Drugs currently used in this pathway of treatment such as Digitalis have adverse toxic effects. This study examines the inhibitory effects of linoleic acid (L.A) on sodium potassium pumps in isolated rat cardiomyocytes using the incorporation of rubidium detected by SEM/EDS technology as the index for sodium-potassium pump activity. Isolated heart cells were incubated in the following treatments: Rb-Krebs buffer without treatment (negative control), Ouabain+ Rb-Krebs buffer (positive control), 10-3 M L.A+ Rb-Krebs buffer, and 10-6 M L.A+ Rb-Krebs buffer. The results established Rb-Krebs as the negative control with a mean of 1.279 +/- 0.075 for Rb % average of 10 replicates with 10 cells analyzed in each replicate. Ouabain + Rb-Krebs was established as the positive control for inhibition of rubidium uptake with a mean value of 0.701 +/- 0.073. It was found that the 10-6 M, and not 10-3 M, concentration of linoleic acid exhibited inhibitory effects of rubidium uptake with a mean value of 0.805 +/- 0.121 and 0.903 +/- 0.095, respectively.

Advisor

Michael Bentley

Committee Member

David Sharlin

Committee Member

Penny Knoblich

Date of Degree

2017

Language

english

Document Type

Thesis

Degree

Master of Science (MS)

Department

Biological Sciences

College

Science, Engineering and Technology

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Biology Commons

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