The Determination of a Threshold Level of Exogenous Methyl Jasmonate Effecting an Increase in Peroxidase Activity in Protoplasts of Avena Sativa
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Hyung Choe
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Methyl jasmonate plays a dual role in plant systems by participating in both developmental and defense mechanisms. Recent studies have shown that methyl jasmonate can specifically alter gene expression, while wounding and specific elicitors can cause a buildup of methyl jasmonate itself. Peroxidase enzymes are ubiquitous to all plant systems and have long been associated with plant stress and defense responses. The objective of this research was to establish a threshold concentration of methyl jasmonate effecting a maximum increase in peroxidase activity less than twenty-four hours after treatment. While previous experiments have explored the activity of methyl jasmonate on peroxidase activity using whole leaf tissue, we developed a system for testing peroxidase activity in oat protoplasts after isolation and subsequent treatment and incubation in a suitable culture media.
The Determination of a Threshold Level of Exogenous Methyl Jasmonate Effecting an Increase in Peroxidase Activity in Protoplasts of Avena Sativa
CSU
Methyl jasmonate plays a dual role in plant systems by participating in both developmental and defense mechanisms. Recent studies have shown that methyl jasmonate can specifically alter gene expression, while wounding and specific elicitors can cause a buildup of methyl jasmonate itself. Peroxidase enzymes are ubiquitous to all plant systems and have long been associated with plant stress and defense responses. The objective of this research was to establish a threshold concentration of methyl jasmonate effecting a maximum increase in peroxidase activity less than twenty-four hours after treatment. While previous experiments have explored the activity of methyl jasmonate on peroxidase activity using whole leaf tissue, we developed a system for testing peroxidase activity in oat protoplasts after isolation and subsequent treatment and incubation in a suitable culture media.
