Gestational Plasma Aldosterone Concentration in SHR and WKY Rats on Normal and Low Sodium Diets
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Penny Knoblich
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
educed perinatal sodium intake attenuates the development of hypertension in the SHR. Stimulation of the renin-angiotensin-aldosterone system by low sodium diets is well documented in non-pregnant rats, but not well documented in pregnant SHR rats. We examined the effect of pregnancy and a low sodium diet on blood pressure and plasma aldosterone concentration in SHR and WKY rats. Blood pressure was measured by the tail cult method prior to and during week 1 of gestation. Rats were mated randomly within their own breed and were placed on either a control sodium diet (0.2% sodium) or a low sodium diet (0.03% sodium). Non-mated rats were placed only on a control sodium diet. Rats were sacrificed by decapitation on day 7 of gestation or at a comparable age (non-pregnant controls), and trunk blood was collected for aldosterone assay. Results: Blood pressures were significantly higher in the SHR than WKY for each comparable data point, but within each breed, blood pressure did not vary significantly with pregnancy or sodium intake. Plasma aldosterone concentrations did not differ significantly between the SHR and WKY breeds undergoing similar treatments, and aldosterone concentration was not increased in either breed during pregnancy (SHR: 26± 5 verses 16± 5 ng/dl; WKY: 21± 5 verses I8± 2 ng/dl). However, the low sodium diet did significantly increase plasma aldosterone (p < 0.05) in both the pregnant SHR and WKY females, (SHR: 16± 5 to 88± 23 ng/dl; WKY: 18± 2 to 85± 14 ng/dl). Conclusion: Gestational plasma aldosterone concentrations are similar in the SHR and WKY rats and are not different than non-pregnant levels but are significantly increased in both rat breeds by sodium restriction.
Gestational Plasma Aldosterone Concentration in SHR and WKY Rats on Normal and Low Sodium Diets
CSU
educed perinatal sodium intake attenuates the development of hypertension in the SHR. Stimulation of the renin-angiotensin-aldosterone system by low sodium diets is well documented in non-pregnant rats, but not well documented in pregnant SHR rats. We examined the effect of pregnancy and a low sodium diet on blood pressure and plasma aldosterone concentration in SHR and WKY rats. Blood pressure was measured by the tail cult method prior to and during week 1 of gestation. Rats were mated randomly within their own breed and were placed on either a control sodium diet (0.2% sodium) or a low sodium diet (0.03% sodium). Non-mated rats were placed only on a control sodium diet. Rats were sacrificed by decapitation on day 7 of gestation or at a comparable age (non-pregnant controls), and trunk blood was collected for aldosterone assay. Results: Blood pressures were significantly higher in the SHR than WKY for each comparable data point, but within each breed, blood pressure did not vary significantly with pregnancy or sodium intake. Plasma aldosterone concentrations did not differ significantly between the SHR and WKY breeds undergoing similar treatments, and aldosterone concentration was not increased in either breed during pregnancy (SHR: 26± 5 verses 16± 5 ng/dl; WKY: 21± 5 verses I8± 2 ng/dl). However, the low sodium diet did significantly increase plasma aldosterone (p < 0.05) in both the pregnant SHR and WKY females, (SHR: 16± 5 to 88± 23 ng/dl; WKY: 18± 2 to 85± 14 ng/dl). Conclusion: Gestational plasma aldosterone concentrations are similar in the SHR and WKY rats and are not different than non-pregnant levels but are significantly increased in both rat breeds by sodium restriction.
