Comparison of Lipoxygenase Isoenzymes Found in Specific Soybean Tissues
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
The purpose of this project was to identify and quantitate the various lipoxygenase (LOX) isoenzymes present in specific soybean tissues. Dififerent varieties of soybeans lacking specific seed lipoxygenase isoenzymes were tested to compare differences in LOX enzymes present in the soybean vegetative tissues. The lipoxygenase isoenzymes were isolated using an electrophoretic technique called isoelectric focusing to separate the isoenzymes by their charges. A specific activity stain was used to identify and quantify the LOX isoenzymes present. The lipoxygenase species present were identified according to their isoelectric points and quantified using a densitometer. This study has shown several key differences among the lipoxygenase species present in the root, stem, and leaf tissues as well as differences in the amount and type of LOX isoenzymes expressed in the control and null seed varieties. Thus, it appears that lipoxygenase expression in the vegetative form is dependent on many factors, including the seed lipoxygenase genes present.
Comparison of Lipoxygenase Isoenzymes Found in Specific Soybean Tissues
The purpose of this project was to identify and quantitate the various lipoxygenase (LOX) isoenzymes present in specific soybean tissues. Dififerent varieties of soybeans lacking specific seed lipoxygenase isoenzymes were tested to compare differences in LOX enzymes present in the soybean vegetative tissues. The lipoxygenase isoenzymes were isolated using an electrophoretic technique called isoelectric focusing to separate the isoenzymes by their charges. A specific activity stain was used to identify and quantify the LOX isoenzymes present. The lipoxygenase species present were identified according to their isoelectric points and quantified using a densitometer. This study has shown several key differences among the lipoxygenase species present in the root, stem, and leaf tissues as well as differences in the amount and type of LOX isoenzymes expressed in the control and null seed varieties. Thus, it appears that lipoxygenase expression in the vegetative form is dependent on many factors, including the seed lipoxygenase genes present.
