Purification Of Actin Capping Protein α – Subunit Isoform Specific Antibodies

Location

CSU

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Marilyn Hart

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Actin is a protein that is vital to muscle contraction and cell motility. Actin is a polymer that possesses two very distinct ends: the pointed end and the barbed end. Capping protein (CP) binds the barbed end. Actin assembly is regulated by a variety of proteins including actin CP. CP is a heterodiraer, composed of an α and β subunit. In vertebrate, the α subunit has three isoforms: α1, α2 and α3, and three beta isoforms: β1, β2 and β3. The α and β isoforms have conserved sequences across vertebrates, suggesting conserved specific functions. Previous research has identified the localization of the β-subunit isoforms and gained information about their function, a isoform specific antibodies were purified using polyclonal antisera, which will be used to determine the cell and tissue specific locations of the α-subunit isoforms of CP.

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Purification Of Actin Capping Protein α – Subunit Isoform Specific Antibodies

CSU

Actin is a protein that is vital to muscle contraction and cell motility. Actin is a polymer that possesses two very distinct ends: the pointed end and the barbed end. Capping protein (CP) binds the barbed end. Actin assembly is regulated by a variety of proteins including actin CP. CP is a heterodiraer, composed of an α and β subunit. In vertebrate, the α subunit has three isoforms: α1, α2 and α3, and three beta isoforms: β1, β2 and β3. The α and β isoforms have conserved sequences across vertebrates, suggesting conserved specific functions. Previous research has identified the localization of the β-subunit isoforms and gained information about their function, a isoform specific antibodies were purified using polyclonal antisera, which will be used to determine the cell and tissue specific locations of the α-subunit isoforms of CP.