Purification Of Actin Capping Protein α – Subunit Isoform Specific Antibodies
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Marilyn Hart
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Actin is a protein that is vital to muscle contraction and cell motility. Actin is a polymer that possesses two very distinct ends: the pointed end and the barbed end. Capping protein (CP) binds the barbed end. Actin assembly is regulated by a variety of proteins including actin CP. CP is a heterodiraer, composed of an α and β subunit. In vertebrate, the α subunit has three isoforms: α1, α2 and α3, and three beta isoforms: β1, β2 and β3. The α and β isoforms have conserved sequences across vertebrates, suggesting conserved specific functions. Previous research has identified the localization of the β-subunit isoforms and gained information about their function, a isoform specific antibodies were purified using polyclonal antisera, which will be used to determine the cell and tissue specific locations of the α-subunit isoforms of CP.
Purification Of Actin Capping Protein α – Subunit Isoform Specific Antibodies
CSU
Actin is a protein that is vital to muscle contraction and cell motility. Actin is a polymer that possesses two very distinct ends: the pointed end and the barbed end. Capping protein (CP) binds the barbed end. Actin assembly is regulated by a variety of proteins including actin CP. CP is a heterodiraer, composed of an α and β subunit. In vertebrate, the α subunit has three isoforms: α1, α2 and α3, and three beta isoforms: β1, β2 and β3. The α and β isoforms have conserved sequences across vertebrates, suggesting conserved specific functions. Previous research has identified the localization of the β-subunit isoforms and gained information about their function, a isoform specific antibodies were purified using polyclonal antisera, which will be used to determine the cell and tissue specific locations of the α-subunit isoforms of CP.
