Nisin Effects on Bacillus Cereus Endospore Germination
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Dorothy Wrigley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
The control of bacterial endospore formers in foods is essential for providing a safe product for consumers. Endospores are resistant structures formed by certain kinds of bacilli. The purpose of this study was to first examine the influence of two gerrainants, L-alanine and inosine, on the Bacillus cereus endospores, and then the impact of nisin on the induction of germination. Nisin is a biopreservative that affects the germination of these spores. The spores were mixed with different concentrations of germinants in the presence or absence of nisin and the germination assessed by changes in absorbance, which occurs as the spore begins germination. The data indicates that both germinants are necessary for good germination. At low concentrations of alanine and high concentrations of inosine, fewer spores germinated in the presence of nisin than without nisin. However, there was more germination of spores at lower concentrations of both germinants when nisin was present than when it was not.
Nisin Effects on Bacillus Cereus Endospore Germination
CSU
The control of bacterial endospore formers in foods is essential for providing a safe product for consumers. Endospores are resistant structures formed by certain kinds of bacilli. The purpose of this study was to first examine the influence of two gerrainants, L-alanine and inosine, on the Bacillus cereus endospores, and then the impact of nisin on the induction of germination. Nisin is a biopreservative that affects the germination of these spores. The spores were mixed with different concentrations of germinants in the presence or absence of nisin and the germination assessed by changes in absorbance, which occurs as the spore begins germination. The data indicates that both germinants are necessary for good germination. At low concentrations of alanine and high concentrations of inosine, fewer spores germinated in the presence of nisin than without nisin. However, there was more germination of spores at lower concentrations of both germinants when nisin was present than when it was not.
