Cloning of ACC Oxidase from Cucumber and Muskmelon
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
James E. Rife
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
The conversion of l-aminocyclopropane carboxylic acid (ACC) into ethyiene is catalyzed by an enzyme known as ACC oxidase. Ethyiene is a plant hormone that regulates many processes in plants, including ripening of the Iruit. In this research, two different fruits with similar gene sequences, cucumber and muskmelon, were studied. The ACC oxidase gene in the muskmelon pulp, the cucumber pulp, and the cucumber leaves was extracted as mRNA, converted into cDNA, and amplified by RT-PCR. It was then cloned into an expression vector and transformed. This provided a readily available source of ACC oxidase so that the enzyme could be physically and kinetically characterized
Cloning of ACC Oxidase from Cucumber and Muskmelon
CSU
The conversion of l-aminocyclopropane carboxylic acid (ACC) into ethyiene is catalyzed by an enzyme known as ACC oxidase. Ethyiene is a plant hormone that regulates many processes in plants, including ripening of the Iruit. In this research, two different fruits with similar gene sequences, cucumber and muskmelon, were studied. The ACC oxidase gene in the muskmelon pulp, the cucumber pulp, and the cucumber leaves was extracted as mRNA, converted into cDNA, and amplified by RT-PCR. It was then cloned into an expression vector and transformed. This provided a readily available source of ACC oxidase so that the enzyme could be physically and kinetically characterized
