Quantifying the Immunoreactivity of Polyclonal IgG and IgY
Location
CSU 253/254/255
Start Date
12-4-2004 1:45 PM
End Date
12-4-2004 3:15 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Marilyn Hart
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Actin, a filament found in the cytoplasm in all eukaryotic cells, contributes to cell shape, cell mobility, and to the organization of certain tissues such as striated muscle. Actin is regulated by a variety of proteins, including actin capping protein (CP). CP is composed of two subunits, an alpha and a beta subunit. In previous studies the beta subunits have been shown to have distinct functions in murine myocardium. The goal is to determine if the alpha subunits, reminiscent of the beta subunits, have similar or distinct functions in cells and tissues. As a first step towards accomplishing this, we will determine the location of the alpha subunits in cells/tissues using antibodies specific for each alpha isoform. The objective of this research was to characterize recently generated chicken anti-alpha 2 IgG and IgY antibodies, quantifying their immonoreactivity. Murine hearts were removed, flash frozen, and the tissue solubilized. The proteins were separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDSPAGE) and transferred to Nitrocellulose (NC) for subsequent Western Blot analysis. The inunobilized proteins were allowed to react with dilutions of the antibodies and visualized with a secondary antibody labeled with alkaline phosphatase. The reactive titers of both the chicken anti-alpha 2 IgG and the chicken anti-alpha 2 IgY antibodies were 10^ providing an initial characterization of the newly generated antibodies and suggesting an approximate working dilution for subsequent studies.
Quantifying the Immunoreactivity of Polyclonal IgG and IgY
CSU 253/254/255
Actin, a filament found in the cytoplasm in all eukaryotic cells, contributes to cell shape, cell mobility, and to the organization of certain tissues such as striated muscle. Actin is regulated by a variety of proteins, including actin capping protein (CP). CP is composed of two subunits, an alpha and a beta subunit. In previous studies the beta subunits have been shown to have distinct functions in murine myocardium. The goal is to determine if the alpha subunits, reminiscent of the beta subunits, have similar or distinct functions in cells and tissues. As a first step towards accomplishing this, we will determine the location of the alpha subunits in cells/tissues using antibodies specific for each alpha isoform. The objective of this research was to characterize recently generated chicken anti-alpha 2 IgG and IgY antibodies, quantifying their immonoreactivity. Murine hearts were removed, flash frozen, and the tissue solubilized. The proteins were separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDSPAGE) and transferred to Nitrocellulose (NC) for subsequent Western Blot analysis. The inunobilized proteins were allowed to react with dilutions of the antibodies and visualized with a secondary antibody labeled with alkaline phosphatase. The reactive titers of both the chicken anti-alpha 2 IgG and the chicken anti-alpha 2 IgY antibodies were 10^ providing an initial characterization of the newly generated antibodies and suggesting an approximate working dilution for subsequent studies.
