Identification of Soybean Lipoxygenase Products by Gas ChromatographyMass Spectroscopy (Gc-Ms)
Location
CSU 285
Start Date
26-4-2005 10:30 AM
End Date
26-4-2005 11:45 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Lipoxygenases (LOXs) are enzymes which catalyze peroxidation of polyunsaturated fatty acids containing at least one cis, cis 1-4 pentadiene moiety to form conjugated diene-hydroperoxides. Soybean seeds contain three LOX isoenzymes while at least five different isoenzymes are in the vegetative tissue. Lipoxygenases have been related to several functions i.e. plant growth, defense mechanisms against pathogens and pests, and lipid metabolism. Given the proposed roles of LOX isoenzymes and the presence of multiple LOX isoenzymes in soybean vegetative tissue, it is likely that individual isoenzymes play specific functions. Do the isoenzymes show differences in which products they form? The complete characterization and comparison of products can give clues about the physiological roles of specific isoenzymes. The basic goal of this project was to develop a GCMS assay to determine the product formed by soybean LOX, and determine the preferred products formed by the different isoenzymes. In this research, linoleic acid and linolenic acid were used as substrates for LOX enzymes to analyze the primary reaction products of LOX in order to see which end of the 1- 4 pentadiene section is used in the reaction. The peroxidation of linoleic acid can take place either at Carbon atom 9 or the carbon atom 13 to form 9-hydroperoxylinoleic acid (9-HPOD) and 13-hydroperoxylinoleic acid (13 HPOD) respectively. Other reactants such as linolenic acid, which has three double bounds, can yield even more products. A GC-MS assay was developed and implemented for analysis of reaction products of LOX.
Identification of Soybean Lipoxygenase Products by Gas ChromatographyMass Spectroscopy (Gc-Ms)
CSU 285
Lipoxygenases (LOXs) are enzymes which catalyze peroxidation of polyunsaturated fatty acids containing at least one cis, cis 1-4 pentadiene moiety to form conjugated diene-hydroperoxides. Soybean seeds contain three LOX isoenzymes while at least five different isoenzymes are in the vegetative tissue. Lipoxygenases have been related to several functions i.e. plant growth, defense mechanisms against pathogens and pests, and lipid metabolism. Given the proposed roles of LOX isoenzymes and the presence of multiple LOX isoenzymes in soybean vegetative tissue, it is likely that individual isoenzymes play specific functions. Do the isoenzymes show differences in which products they form? The complete characterization and comparison of products can give clues about the physiological roles of specific isoenzymes. The basic goal of this project was to develop a GCMS assay to determine the product formed by soybean LOX, and determine the preferred products formed by the different isoenzymes. In this research, linoleic acid and linolenic acid were used as substrates for LOX enzymes to analyze the primary reaction products of LOX in order to see which end of the 1- 4 pentadiene section is used in the reaction. The peroxidation of linoleic acid can take place either at Carbon atom 9 or the carbon atom 13 to form 9-hydroperoxylinoleic acid (9-HPOD) and 13-hydroperoxylinoleic acid (13 HPOD) respectively. Other reactants such as linolenic acid, which has three double bounds, can yield even more products. A GC-MS assay was developed and implemented for analysis of reaction products of LOX.
