Effects of Stress During Sporulation on the Germination Rate of Spores

Location

CSU 255

Start Date

26-4-2005 10:00 AM

End Date

26-4-2005 12:00 PM

Student's Major

College of Science, Engineering and Technology

Student's College

Science, Engineering and Technology

Mentor's Name

Dorothy Wrigley

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Bacillus cereiis is a spore forming bacterium that can cause food-home disease. It is very important to control this bacterium in food in order to prevent disease and spoilage. Control is difficult because of the ability of the organisms to make endospores which are more resistant to treatments to eliminate bacteria than the vegetative cells. This study is examining the effects of stress during spore formation on the germination of the endospores. It is hypothesized that stress during formation may enhance the resistance of the endospores to various treatments used to control its growth such as nisin addition and heat treatments. Nisin is a biopreservative. Spores were formed under stress of temperature shift, presence of sub-lethal concentrations of nisin, and pH differences. To date the data has shown that sub-lethal nisin stress does affect the germination slightly. In addition, I have confirmed research that indicates that B. cereus will not form spores at pH 4. Additional data will be presented.

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Apr 26th, 10:00 AM Apr 26th, 12:00 PM

Effects of Stress During Sporulation on the Germination Rate of Spores

CSU 255

Bacillus cereiis is a spore forming bacterium that can cause food-home disease. It is very important to control this bacterium in food in order to prevent disease and spoilage. Control is difficult because of the ability of the organisms to make endospores which are more resistant to treatments to eliminate bacteria than the vegetative cells. This study is examining the effects of stress during spore formation on the germination of the endospores. It is hypothesized that stress during formation may enhance the resistance of the endospores to various treatments used to control its growth such as nisin addition and heat treatments. Nisin is a biopreservative. Spores were formed under stress of temperature shift, presence of sub-lethal concentrations of nisin, and pH differences. To date the data has shown that sub-lethal nisin stress does affect the germination slightly. In addition, I have confirmed research that indicates that B. cereus will not form spores at pH 4. Additional data will be presented.