Characterization of Functional Abnormalities in Hearts of Transenic Mice Expressing Forms of Actin Capping Protein Defective in Attracting Thin Filaments to Z Lines
Location
CSU 255
Start Date
26-4-2005 10:00 AM
End Date
26-4-2005 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Marilyn C. Hart
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
In striated muscle, the barbed ends of thin filaments are attached to Z lines. Biochemical and cell biological studies suggests that actin capping protein (CP) mediates this attachment by binding the barbed ends of the actin filaments. Defective interaction between CP and the actin thin filaments causes major structural defects in sarcomere organization and leads to cardiac hypertrophy and lethality. The goal of this research is to understand how the defects in the attachment of thin filaments to Z-lines affects the function of the heart. In preliminary studies in the laboratory of Dr. Marilyn Hart, blood pressure was measured in transgenic and wild type mice using an indirect tail pressure assay. The transgenic mice had a lower systolic pressure and an increased heart rate compared to wild type mice. In our present study, we have confirmed and expanded these preliminary results using electrocardiogram (ECG) and direct pressure measurements. Briefly, wildtype and transgenic mice, approximately six months of age, were anesthetized, ECG measurements determined, followed by cardiac catherization to analyze blood pressure directly. After these studies, the mice were allowed to stabilize for thirty minutes and a final ECG recorded. Indirect measurements were compared to direct measurements and an accuracy value calculated.
Characterization of Functional Abnormalities in Hearts of Transenic Mice Expressing Forms of Actin Capping Protein Defective in Attracting Thin Filaments to Z Lines
CSU 255
In striated muscle, the barbed ends of thin filaments are attached to Z lines. Biochemical and cell biological studies suggests that actin capping protein (CP) mediates this attachment by binding the barbed ends of the actin filaments. Defective interaction between CP and the actin thin filaments causes major structural defects in sarcomere organization and leads to cardiac hypertrophy and lethality. The goal of this research is to understand how the defects in the attachment of thin filaments to Z-lines affects the function of the heart. In preliminary studies in the laboratory of Dr. Marilyn Hart, blood pressure was measured in transgenic and wild type mice using an indirect tail pressure assay. The transgenic mice had a lower systolic pressure and an increased heart rate compared to wild type mice. In our present study, we have confirmed and expanded these preliminary results using electrocardiogram (ECG) and direct pressure measurements. Briefly, wildtype and transgenic mice, approximately six months of age, were anesthetized, ECG measurements determined, followed by cardiac catherization to analyze blood pressure directly. After these studies, the mice were allowed to stabilize for thirty minutes and a final ECG recorded. Indirect measurements were compared to direct measurements and an accuracy value calculated.
