Visualization of the Microvasculature Structure of the Rat Sciatic Nerve

Location

CSU 253/4/5

Start Date

23-4-2007 1:00 PM

End Date

23-4-2007 3:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Michael Bentley

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

The vascular supply to the peripheral nerves is important for axon maintenance and survival. Little information is known about the architecture of the microvascuiature and capillary beds of peripheral nerves. The microvascuiature architecture of sciatic nerves from male adult Wistar Kyoto rats were visualized by scanning electron microscopy. To visualize the vessels, a relatively new PU4ii polyurethane-based casting resin was infused into the aorta. The sciatic nerves were dissected after the resin completely polymerized. The nerves were placed in concentrated potassium chloride solution. The resulting casts were critical point dried, sputter coated with gold palladium and analyzed via scanning electron microscopy. The arterial supply and venous drainage were visible running parallel to the sciatic nerve. Capillary networks extended between the longitudinally arranged arterioles and venuoles. The arrangement may have relevance in experimental neuropathic conditions such as diabetes mellitus.

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Apr 23rd, 1:00 PM Apr 23rd, 3:00 PM

Visualization of the Microvasculature Structure of the Rat Sciatic Nerve

CSU 253/4/5

The vascular supply to the peripheral nerves is important for axon maintenance and survival. Little information is known about the architecture of the microvascuiature and capillary beds of peripheral nerves. The microvascuiature architecture of sciatic nerves from male adult Wistar Kyoto rats were visualized by scanning electron microscopy. To visualize the vessels, a relatively new PU4ii polyurethane-based casting resin was infused into the aorta. The sciatic nerves were dissected after the resin completely polymerized. The nerves were placed in concentrated potassium chloride solution. The resulting casts were critical point dried, sputter coated with gold palladium and analyzed via scanning electron microscopy. The arterial supply and venous drainage were visible running parallel to the sciatic nerve. Capillary networks extended between the longitudinally arranged arterioles and venuoles. The arrangement may have relevance in experimental neuropathic conditions such as diabetes mellitus.

Recommended Citation

Witthaus, Michael W. and Adam W. Sudbeck. "Visualization of the Microvasculature Structure of the Rat Sciatic Nerve." Undergraduate Research Symposium, Mankato, MN, April 23, 2007.
https://cornerstone.lib.mnsu.edu/urs/2007/poster-session-A/1