Vascular Architecture of the Liver in SHR and WKY Rats
Location
CSU 253/4/5
Start Date
23-4-2007 1:00 PM
End Date
23-4-2007 3:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
The liver is a highly complex vascular organ containing an intricate network of arteries, arterioles, portal veins, veins, venules, and capillaries. This complex network could change in situations related to vascular disease such as hypertension. We examined the vasculature in rat livers by scanning electron microscopy. The tissue was prepared by perfusing either Mercox resin or polyurethane resin into the vasculature. Once the resin polymerized, each liver was placed in concentrated potassium hydroxide to corrode the tissue from the vascular casts. The casts were critical point dried, sputter coated with gold-palladium, and viewed by scanning electron microscopy. Examination of the vascular architecture showed the circulatory patterns in the various lobes of the liver. The capillaries from the arterial and portal venous supply were continuous with the venous drainage. The information gathered in this study may aid ongoing research in hypertension studies and future studies involving liver regeneration.
Vascular Architecture of the Liver in SHR and WKY Rats
CSU 253/4/5
The liver is a highly complex vascular organ containing an intricate network of arteries, arterioles, portal veins, veins, venules, and capillaries. This complex network could change in situations related to vascular disease such as hypertension. We examined the vasculature in rat livers by scanning electron microscopy. The tissue was prepared by perfusing either Mercox resin or polyurethane resin into the vasculature. Once the resin polymerized, each liver was placed in concentrated potassium hydroxide to corrode the tissue from the vascular casts. The casts were critical point dried, sputter coated with gold-palladium, and viewed by scanning electron microscopy. Examination of the vascular architecture showed the circulatory patterns in the various lobes of the liver. The capillaries from the arterial and portal venous supply were continuous with the venous drainage. The information gathered in this study may aid ongoing research in hypertension studies and future studies involving liver regeneration.
Recommended Citation
Mathwig, Charalette and Ken Willaert. "Vascular Architecture of the Liver in SHR and WKY Rats." Undergraduate Research Symposium, Mankato, MN, April 23, 2007.
https://cornerstone.lib.mnsu.edu/urs/2007/poster-session-B/4
