Does Maternal Aldosterone Regulate Placental 11-Beta Hydroxysteroid Dehydrogenase Expression in Rats?

Location

CSU 253/4/5

Start Date

23-4-2007 1:00 PM

End Date

23-4-2007 3:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Theresa Salerno

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Second Mentor's Name

James Rife

Second Mentor's Department

Chemistry and Geology

Second Mentor's College

Science, Engineering and Technology

Third Mentor's Name

Penny Knoblich

Third Mentor's Deparment

Biological Sciences

Third Mentor's College

Science, Engineering and Technology

Description

The aim of this project was to investigate whether maternal aidosterone levels regulate the expression of 11- beta hydroxysteroid dehydrogenase (11-pHSD) in the rat placenta. Two isoforms of this enzyme, 11-pHSD type I and type n, have been reported. It has been proposed that in the placenta, these enzymes protect the fetus from the maternal glucocorticoids. The purpose of this project was to measure the levels of 11-pHSD type I and type n mRNAs in the placenta of rats which have different maternal aidosterone levels. In this project, one set of rats had their adrenal medulla, the aidosterone producing layer, destroyed by freezing. The control group of rats had a sham operation. These surgeries were performed by Dr. Knoblich's research group. Placenta from both pregnant experimental and control Wistar-Kyoto (WKY) and Spontaneously Hypertensive rats (SHR) were collected and frozen in liquid nitrogen at day 21 of term. RNA was isolated from the placenta and was used to synthesize cDNAs using RT-PCR. Selective PGR primers were used to specifically amplify each of the two isoenzyme cDNAs in separate experiments. PCR products were quantified relative to an internal standard 18S RNA. The method was optimized to obtain similar amounts of internal standard and 11-pHSD cDNAs in the linear range of the amplification curves.

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Apr 23rd, 1:00 PM Apr 23rd, 3:00 PM

Does Maternal Aldosterone Regulate Placental 11-Beta Hydroxysteroid Dehydrogenase Expression in Rats?

CSU 253/4/5

The aim of this project was to investigate whether maternal aidosterone levels regulate the expression of 11- beta hydroxysteroid dehydrogenase (11-pHSD) in the rat placenta. Two isoforms of this enzyme, 11-pHSD type I and type n, have been reported. It has been proposed that in the placenta, these enzymes protect the fetus from the maternal glucocorticoids. The purpose of this project was to measure the levels of 11-pHSD type I and type n mRNAs in the placenta of rats which have different maternal aidosterone levels. In this project, one set of rats had their adrenal medulla, the aidosterone producing layer, destroyed by freezing. The control group of rats had a sham operation. These surgeries were performed by Dr. Knoblich's research group. Placenta from both pregnant experimental and control Wistar-Kyoto (WKY) and Spontaneously Hypertensive rats (SHR) were collected and frozen in liquid nitrogen at day 21 of term. RNA was isolated from the placenta and was used to synthesize cDNAs using RT-PCR. Selective PGR primers were used to specifically amplify each of the two isoenzyme cDNAs in separate experiments. PCR products were quantified relative to an internal standard 18S RNA. The method was optimized to obtain similar amounts of internal standard and 11-pHSD cDNAs in the linear range of the amplification curves.

Recommended Citation

Achu, Emmanuel and Justin Rocheleau. "Does Maternal Aldosterone Regulate Placental 11-Beta Hydroxysteroid Dehydrogenase Expression in Rats?." Undergraduate Research Symposium, Mankato, MN, April 23, 2007.
https://cornerstone.lib.mnsu.edu/urs/2007/poster-session-B/5