Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.

Location

CSU Ballroom

Start Date

21-4-2008 1:00 PM

End Date

21-4-2008 3:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Geoffrey M. Goellner

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

In the early 1990s scientists from all over the globe embarked on a journey to unlock the human genome, to find out how many genes lie twisting and tangling within our chromosomes. Nearly ten years later, the daunting task was complete; the 3 billion base pairs within our double stranded DNA had been sequenced. Now, an equally immense task presents itself for scientists- to discover the cellular function of all of these hitherto unknown genes. In this regard, we have chose􀀓 to characterize the function of an unknown gene­Kiaal 946. This gene is of interest to us because it has a polyglutamine tract within its primary amino acid sequence, and is likely expressed in the nervous system (several other polyglutamine proteins underlie a number of severe neurodegenerative disorders such as Huntington Disease). As a first step in characterizing the cellular function of KIAA1946, we have successfully cloned it into pEGFPNl- a special vector that helps "tag" our novel protein with a fluorescent tracer called GFP (Green Fluorescent Protein). Our next step, will be to transfect our KIAA1946-GFP fusion protein vector into tissue culture cells, and assay (using fluorescence microscopy) the normal cellular localization ofKlAA1946. These data represent a first step in deciphering the normal cellular function of Kiaa1946.

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Apr 21st, 1:00 PM Apr 21st, 3:00 PM

Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.

CSU Ballroom

In the early 1990s scientists from all over the globe embarked on a journey to unlock the human genome, to find out how many genes lie twisting and tangling within our chromosomes. Nearly ten years later, the daunting task was complete; the 3 billion base pairs within our double stranded DNA had been sequenced. Now, an equally immense task presents itself for scientists- to discover the cellular function of all of these hitherto unknown genes. In this regard, we have chose􀀓 to characterize the function of an unknown gene­Kiaal 946. This gene is of interest to us because it has a polyglutamine tract within its primary amino acid sequence, and is likely expressed in the nervous system (several other polyglutamine proteins underlie a number of severe neurodegenerative disorders such as Huntington Disease). As a first step in characterizing the cellular function of KIAA1946, we have successfully cloned it into pEGFPNl- a special vector that helps "tag" our novel protein with a fluorescent tracer called GFP (Green Fluorescent Protein). Our next step, will be to transfect our KIAA1946-GFP fusion protein vector into tissue culture cells, and assay (using fluorescence microscopy) the normal cellular localization ofKlAA1946. These data represent a first step in deciphering the normal cellular function of Kiaa1946.

Recommended Citation

Jensen, Kyle. "Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.." Undergraduate Research Symposium, Mankato, MN, April 21, 2008.
https://cornerstone.lib.mnsu.edu/urs/2008/poster-session-B/10