Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.
Location
CSU Ballroom
Start Date
21-4-2008 1:00 PM
End Date
21-4-2008 3:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Geoffrey M. Goellner
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
In the early 1990s scientists from all over the globe embarked on a journey to unlock the human genome, to find out how many genes lie twisting and tangling within our chromosomes. Nearly ten years later, the daunting task was complete; the 3 billion base pairs within our double stranded DNA had been sequenced. Now, an equally immense task presents itself for scientists- to discover the cellular function of all of these hitherto unknown genes. In this regard, we have chose to characterize the function of an unknown geneKiaal 946. This gene is of interest to us because it has a polyglutamine tract within its primary amino acid sequence, and is likely expressed in the nervous system (several other polyglutamine proteins underlie a number of severe neurodegenerative disorders such as Huntington Disease). As a first step in characterizing the cellular function of KIAA1946, we have successfully cloned it into pEGFPNl- a special vector that helps "tag" our novel protein with a fluorescent tracer called GFP (Green Fluorescent Protein). Our next step, will be to transfect our KIAA1946-GFP fusion protein vector into tissue culture cells, and assay (using fluorescence microscopy) the normal cellular localization ofKlAA1946. These data represent a first step in deciphering the normal cellular function of Kiaa1946.
Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.
CSU Ballroom
In the early 1990s scientists from all over the globe embarked on a journey to unlock the human genome, to find out how many genes lie twisting and tangling within our chromosomes. Nearly ten years later, the daunting task was complete; the 3 billion base pairs within our double stranded DNA had been sequenced. Now, an equally immense task presents itself for scientists- to discover the cellular function of all of these hitherto unknown genes. In this regard, we have chose to characterize the function of an unknown geneKiaal 946. This gene is of interest to us because it has a polyglutamine tract within its primary amino acid sequence, and is likely expressed in the nervous system (several other polyglutamine proteins underlie a number of severe neurodegenerative disorders such as Huntington Disease). As a first step in characterizing the cellular function of KIAA1946, we have successfully cloned it into pEGFPNl- a special vector that helps "tag" our novel protein with a fluorescent tracer called GFP (Green Fluorescent Protein). Our next step, will be to transfect our KIAA1946-GFP fusion protein vector into tissue culture cells, and assay (using fluorescence microscopy) the normal cellular localization ofKlAA1946. These data represent a first step in deciphering the normal cellular function of Kiaa1946.
Recommended Citation
Jensen, Kyle. "Cloning and Initial Functional Characterization of Unknown Gene KIAA1946.." Undergraduate Research Symposium, Mankato, MN, April 21, 2008.
https://cornerstone.lib.mnsu.edu/urs/2008/poster-session-B/10
